Qian Hengwei, Wang Lulu, Ma Xianliang, Yi Xingling, Wang Baoshan, Liang Wenxing
College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China.
College of Life Sciences, Shandong Normal University, Jinan, China.
Front Microbiol. 2021 Feb 10;12:623735. doi: 10.3389/fmicb.2021.623735. eCollection 2021.
Protein lysine 2-hydroxyisobutyrylation (K ), a new type of post-translational modification, occurs in histones and non-histone proteins and plays an important role in almost all aspects of both eukaryotic and prokaryotic living cells. , a soil-borne fungal pathogen, can cause disease in more than 150 plants. However, little is currently known about the functions of K in this plant pathogenic fungus. Here, we report a systematic analysis of 2-hydroxyisobutyrylated proteins in . In this study, 3782 K sites in 1299 proteins were identified in . The bioinformatics analysis showed that 2-hydroxyisobutyrylated proteins are involved in different biological processes and functions and are located in diverse subcellular localizations. The enrichment analysis revealed that K participates in a variety of pathways, including the ribosome, oxidative phosphorylation, and proteasome pathways. The protein interaction network analysis showed that 2-hydroxyisobutyrylated protein complexes are involved in diverse interactions. Notably, several 2-hydroxyisobutyrylated proteins, including three kinds of protein kinases, were involved in the virulence or conidiation of , suggesting that K plays regulatory roles in pathogenesis. Moreover, our study shows that there are different K levels of in conidial and mycelial stages. These findings provide evidence of K in , an important filamentous plant pathogenic fungus, and serve as a resource for further exploration of the potential functions of K in species and other filamentous pathogenic fungi.
蛋白质赖氨酸2-羟基异丁酰化（K ）是一种新型的翻译后修饰，存在于组蛋白和非组蛋白中，在真核生物和原核生物活细胞的几乎所有方面都发挥着重要作用。 是一种土传真菌病原体，可导致150多种植物发病。然而，目前对这种植物病原真菌中K 的功能了解甚少。在此，我们报告了对 中2-羟基异丁酰化蛋白质的系统分析。在本研究中，在 中鉴定出1299个蛋白质中的3782个K 位点。生物信息学分析表明，2-羟基异丁酰化蛋白质参与不同的生物学过程和功能，且定位于不同的亚细胞位置。富集分析显示，K 参与多种途径，包括核糖体、氧化磷酸化和蛋白酶体途径。蛋白质相互作用网络分析表明，2-羟基异丁酰化蛋白复合物参与多种相互作用。值得注意的是，几种2-羟基异丁酰化蛋白质，包括三种蛋白激酶，参与了 的毒力或分生孢子形成，这表明K 在发病机制中起调节作用。此外，我们的研究表明， 在分生孢子和菌丝体阶段存在不同的K 水平。这些发现为重要的丝状植物病原真菌 中K 的存在提供了证据，并为进一步探索K 在 物种和其他丝状病原真菌中的潜在功能提供了资源。
