Elliott C T, Francis K S, Shortt H D, McCaughey W J
Drug Residue Laboratory, Department of Agriculture for Northern Ireland, Belfast, UK.
Analyst. 1995 Jun;120(6):1827-30. doi: 10.1039/an9952001827.
The performance of three conventional enzyme and radioimmunoassays routinely used to detect residues of anabolic steroids in cattle sera were compared with dissociation enhanced lanthanide fluorescence immunoassay (DELFIA) kits designed for the hospital market. Slight modifications to the kit reagents were required for the analysis of bovine sera. Owing to the large sample volumes used in conventional assays, detection limits were generally better than those obtained with DELFIA kits, however, assay reproducibility was enhanced using the DELFIA technology. Comparison of sera obtained from cattle implanted with anabolic steroids revealed a good correlation between alternate methods (r2 from 0.91 to 0.97). The DELFIA kits offer a faster method for measuring estradiol, progesterone and testosterone with adequate sensitivity and in a safer environment than that encountered using radioimmunoassays.
将常规用于检测牛血清中合成代谢类固醇残留的三种酶免疫测定法和放射免疫测定法的性能，与为医院市场设计的解离增强镧系元素荧光免疫测定法（DELFIA）试剂盒进行了比较。分析牛血清时需要对试剂盒试剂进行轻微修改。由于常规检测使用的样本量较大，其检测限通常优于使用DELFIA试剂盒获得的检测限，然而，使用DELFIA技术可提高检测的重现性。对植入合成代谢类固醇的牛所获得的血清进行比较，结果显示不同方法之间具有良好的相关性（r2为0.91至0.97）。DELFIA试剂盒提供了一种比放射免疫测定法更快的方法来测量雌二醇、孕酮和睾酮，具有足够的灵敏度，且检测环境更安全。
