Department of Basic Science, Faculty of Dentistry, Kufa University, Najaf, Iraq.
Department of Oral Diagnosis, College of Dentistry, University of Basrah, Basrah, Iraq.
J Med Life. 2022 Jan;15(1):89-97. doi: 10.25122/jml-2021-0184.
This study aimed to compare the antimicrobial effect of an aqueous extract Red Roselle calyx (RE), Chlorhexidine (CH), Amoxicillin-clavulanic acid (ACA), Tetracycline (Tet), and Metronidazole (Met)on (S. mutans), () and () bacteria. The bacterial inhibition zones (BIZ)of the RE (25, 50, 75, 100) mg/ml and CH solutions (0.2%, 2%) were determined using the agar well diffusion method. Additionally, the susceptibility of the tested bacteria against (30 μg) of standard antibiotics of ACA, Tet, and Met was examined. The bacterial minimum inhibitory concentration (MIC) was measured using the Broth Micro dilution method (BMDM). All tests were carried out in triplicates, and water was considered the negative control. For , the RE at 50 mg/ml or above concentrations displayed higher BIZ than 0.2% CH. 100 mg/ml of RE recorded a greater BIZ than the 2% CH. The greater BIZ against S. mutans was recorded by Tet. A comparable effect was found with 0.2% CH (75, 100) mg/ml of the RE against . Greater BIZ for and were reported for 100 mg/ml RE compared to the Tet and Met RE at 100 mg/ml inhibited the growth in a zone size comparable to the CH (0.2%, 2%).The RE with 50,100 mg/ml concentrations showed comparable antimicrobial effect to 0.2%, 2% concentrations of CH, respectively. As an herbal substitute for commercial disinfectants, the RE can be considered an effective final endodontic irrigant and dental mouthwash.
本研究旨在比较红玫瑰茄水提物（RE）、洗必泰（CH）、阿莫西林克拉维酸（ACA）、四环素（Tet）和甲硝唑（Met）对（S. mutans）、（）和（）细菌的抗菌效果。采用琼脂孔扩散法测定 RE（25、50、75、100）mg/ml 和 CH 溶液（0.2%、2%）的抑菌圈（BIZ）。此外，还检测了测试细菌对 ACA、Tet 和 Met 的标准抗生素（30μg）的敏感性。采用肉汤微量稀释法（BMDM）测定细菌最小抑菌浓度（MIC）。所有试验均重复 3 次，水为阴性对照。对于，RE 在 50mg/ml 或更高浓度下显示出比 0.2% CH 更高的 BIZ。100mg/ml 的 RE 记录的 BIZ 大于 2% CH。Tet 对 S. mutans 的抑制作用更大。RE 对 0.2%、100mg/ml 的 BIZ 与 0.2% CH 相似。与 Tet 和 Met 相比，RE 对 100mg/ml 对 和 的抑制作用更大。RE 对 100mg/ml 的抑菌作用大于 Tet 和 Met 对 100mg/ml 的抑菌作用。RE 浓度为 50、100mg/ml 时，抗菌效果与 CH 浓度为 0.2%、2%时相当。作为商业消毒剂的草药替代品，RE 可作为有效的根管冲洗剂和口腔漱口水。
