1%玫瑰茄花纳米乳液提取物（玫瑰茄）对正畸微型种植体周围种植体周围炎相关细菌的抗菌活性：一项体外研究。
Antibacterial activity of 1 % Roselle flower nano-emulsion extract (Hibiscus sabdariffa) against peri-implantitis-related bacteria on orthodontic mini-implants: An in vitro study.
作者信息
Abdillah Ridhofar Akbar Khusnul, Budhy Theresia Indah, Narmada Ida Bagus, Ardani I Gusti Aju Wahju, Ramadhani Nastiti Faradilla, Sitalaksmi Ratri Maya, Luthfi Muhammad, Ulfa Ninik Mas, Nugraha Albertus Putera, Tengku Ahmad Noor Tengku Natasha Eleena Binti, Nugraha Alexander Patera
机构信息
Graduate Student of Dental Health Science, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.
Oral and Maxillofacial Pathology Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.
出版信息
J Oral Biol Craniofac Res. 2025 Jan-Feb;15(1):170-177. doi: 10.1016/j.jobcr.2024.12.017. Epub 2025 Jan 11.
OBJECTIVE
The orthodontic mini-implant (OMI) failure often occurs due to the accumulation of peri-implantitis bacteria surrounding it, which results in a stable, resistant form of absolute skeletal anchorage during orthodontic treatment. Administering doxycycline may be the solution, but long-term side effects result in antibiotic resistance. Roselle flowers (Hibiscus sabdariffa) possess beneficial active phytochemical substances, which may have potential as an OMI peri-implantitis alternative therapy. This study investigates the antibacterial activity of 1 % Roselle flower (H. sabdarifa) nanoemulsion (NE) extract (1 % RNE) toward peri-implantitis bacteria in OMIs.
METHODS
A phytochemical analysis of 1 % RNE was carried out to examine the active substances possessed in it, such as flavonoids, quinone, saponin, alkaloids, tannins, terpenoids, and steroids. Nanoemulsion characterization was carried out using a particle size analyzer (PSA). The antibacterial activity of 1 % RNE toward Prevotella intermedia (Pi), Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans (Aa), and Fusobacterium nucleatum (Fn) was carried out to determine the minimum inhibitory concentration, minimum bactericidal concentration, and inhibitory zone compared with doxycycline as a positive control.
RESULTS
Roselle flower NE extract (1 %) possessed flavonoids, quinone, saponin, alkaloids, tannins, terpenoids, and steroids positively. The PSA showed that the 1 % RNE had a size of 98.13 d nm. The antibacterial activity of 1 % RNE against Aa, Pg, Pi, and Fn bacteria at 3.125 % showed significant differences (p < 0.05). The antibacterial activity of 1 % RNE toward peri-implantitis bacteria is lower than that of doxycycline.
CONCLUSION
Roselle flower NE extract (1 %) has antibacterial activity against peri-implantitis bacteria at a concentration of 3.125 %.
目的
正畸微型种植体（OMI）失败常因周围种植体周炎细菌的积聚所致，这会影响正畸治疗期间绝对骨锚固的稳定性和抗性。服用强力霉素可能是一种解决办法，但长期副作用会导致抗生素耐药性。玫瑰茄花（木槿）含有有益的活性植物化学物质，可能作为OMI种植体周炎的替代疗法。本研究调查1%玫瑰茄花（木槿）纳米乳剂（NE）提取物（1%RNE）对OMIs中种植体周炎细菌的抗菌活性。
方法
对1%RNE进行植物化学分析，以检测其中所含的活性物质，如黄酮类、醌类、皂苷、生物碱、单宁、萜类和甾体类。使用粒度分析仪（PSA）对纳米乳剂进行表征。将1%RNE对中间普氏菌（Pi）、牙龈卟啉单胞菌（Pg）、伴放线聚集杆菌（Aa）和具核梭杆菌（Fn）的抗菌活性与作为阳性对照的强力霉素进行比较，以确定最低抑菌浓度、最低杀菌浓度和抑菌圈。
结果
玫瑰茄花NE提取物（1%）含有黄酮类、醌类、皂苷、生物碱、单宁、萜类和甾体类。PSA显示1%RNE的粒径为98.13 d nm。1%RNE在3.125%浓度下对Aa、Pg、Pi和Fn细菌的抗菌活性显示出显著差异（p < 0.05）。1%RNE对种植体周炎细菌的抗菌活性低于强力霉素。
结论
玫瑰茄花NE提取物（1%）在浓度为3.125%时对种植体周炎细菌具有抗菌活性。
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