Kim Dan-Bi, Jung Young Sung, Nam Tae Gyu, Lee Sanghee, Yoo Miyoung
Korea Food Research Institute, Wanju-gun, Jeollabuk-do 55365 Republic of Korea.
Major of Food Science and Biotechnology, Division of Bioconvergence, Kyonggi University, Suwon, 16227 Republic of Korea.
Food Sci Biotechnol. 2022 Jan 25;31(2):165-174. doi: 10.1007/s10068-021-01024-5. eCollection 2022 Feb.
This study was designed to determine the residual trichothecene mycotoxins in cereal samples. The optimal solvent for extraction was 84% (v/v) aqueous acetonitrile with 1% (v/v) formic acid. The best performing clean-up method was dispersive-solid phase with a mixture octadecyl silica and primary-secondary amine. The recoveries for the studied mycotoxins ranged from 83.3 to 92.8%. The methodology was successfully applied for monitoring 100 cereal samples obtained from a Korean market. The bean sample were found to be co-contamination with deoxynivalenol and HT-2 toxin. Deoxynivalenol possessed the highest detection freauency (4/100) and amount (727.38 µg/kg) among the trichothecene mycotoxins. The hazard index was less than 1.0 for all the observed mycotoxins in all cereal samples except one white rice sample (1.2681). This results indicated that periodic risk assessments of trichothecene mycotoxin through cereal intake are necessary for the health and safety.
本研究旨在测定谷物样品中残留的单端孢霉烯族霉菌毒素。最佳提取溶剂为含1%（v/v）甲酸的84%（v/v）乙腈水溶液。最佳净化方法是使用十八烷基硅胶和伯仲胺混合物的分散固相萃取法。所研究霉菌毒素的回收率在83.3%至92.8%之间。该方法成功应用于监测从韩国市场采集的100份谷物样品。发现豆类样品中脱氧雪腐镰刀菌烯醇和HT-2毒素存在共污染。在单端孢霉烯族霉菌毒素中，脱氧雪腐镰刀菌烯醇的检出频率最高（4/100），含量也最高（727.38µg/kg）。除一份白米样品（1.2681）外，所有谷物样品中观察到的所有霉菌毒素的危害指数均小于1.0。该结果表明，为了健康和安全，有必要通过谷物摄入对单端孢霉烯族霉菌毒素进行定期风险评估。
