Filizay Mehmet Caner, Gokcinar Nesrin Buyuktortop, Sahinturk Varol, Oner Setenay, Kacar Sedat, Onaran Zafer, Yumusak Erhan
Department of Ophthalmology, Eskisehir State Hospital, Eskisehir, Turkey.
Department of Ophthalmology, Kirikkale University Faculty of Medicine, Kirikkale, Turkey.
Beyoglu Eye J. 2019 Aug 5;4(2):55-61. doi: 10.14744/bej.2019.51523. eCollection 2019.
Acute exposure to ultraviolet B radiation can cause photokeratitis. Retinol palmitate (RP) is known to have antioxidant properties and improve corneal healing. The aim of this study was to evaluate the effect of topical RP against phototoxic keratitis in rats.
A total of 14 male Wistar Albino rats were exposed to 1 J/cm dose of 311 nm ultraviolet B radiation. The subjects were then divided into 4 study groups using the right and left eye: The RP-5 group (n=7) received topical 250 IU/g RP ointment and the Sham-5 group (n=7) received only the vehicle base component of the ointment 5 minutes after the exposure. The RP-120 group (n=7) received topical RP and the Sham-120 group (n=7) received the vehicle alone 120 minutes after the exposure. The eyes were enucleated 24 hours after the exposure and stained with hematoxylin and eosin for histopathological examination and a terminal deoxynucleotidyl transferase dUTP nick end labeling assay to test for apoptosis.
There was no statistically significant difference between the mean corneal epithelial thickness of the RP-5 group and the Sham-5 group (p=0.369). Furthermore, there was no significant difference between the RP-120 and the Sham-120 groups (p=0.765). The timing of the administration of RP resulted in no significant difference in the mean corneal epithelial thickness (p=0.608). Apoptotic cell count scores were not significantly different between corneas that received RP and those who received only the vehicle (p=0.530, p=0.107).
Topical administration of a single dose of RP was not superior to the use of the vehicle base alone in a photokeratitis model produced using 1 J/cm of narrowband ultraviolet radiation in rats.
急性暴露于紫外线B辐射可导致光性角膜炎。已知视黄醇棕榈酸酯（RP）具有抗氧化特性并能促进角膜愈合。本研究的目的是评估局部应用RP对大鼠光毒性角膜炎的影响。
总共14只雄性Wistar白化大鼠暴露于1 J/cm剂量的311 nm紫外线B辐射。然后使用右眼和左眼将受试者分为4个研究组：RP - 5组（n = 7）在暴露后5分钟接受局部250 IU/g RP软膏，假手术 - 5组（n = 7）仅接受软膏的赋形剂基础成分。RP - 120组（n = 7）在暴露后120分钟接受局部RP，假手术 - 120组（n = 7）仅接受赋形剂。暴露24小时后摘除眼球，用苏木精和伊红染色进行组织病理学检查，并进行末端脱氧核苷酸转移酶dUTP缺口末端标记测定以检测细胞凋亡。
RP - 5组和假手术 - 5组的平均角膜上皮厚度之间无统计学显著差异（p = 0.369）。此外，RP - 120组和假手术 - 120组之间也无显著差异（p = 0.765）。RP给药时间对平均角膜上皮厚度无显著差异（p = 0.608）。接受RP的角膜与仅接受赋形剂的角膜之间的凋亡细胞计数评分无显著差异（p = 0.530，p = 0.107）。
在使用1 J/cm窄带紫外线辐射建立的大鼠光性角膜炎模型中，单次局部应用RP并不优于单独使用赋形剂。
