Odaka Akito, Toshida Hiroshi, Ohta Toshihiko, Tabuchi Nobuhito, Koike Daisuke, Suto Chikako, Murakami Akira
Human and Environmental Safety Evaluation Center, Lion Corporation, Kanagawa, Japan.
Clin Ophthalmol. 2012;6:1585-93. doi: 10.2147/OPTH.S35584. Epub 2012 Oct 1.
We examined the efficacy of retinol palmitate (VApal) for dry eyes using dry eye model rabbits whose lacrimal glands were resected.
After alkaline injury on keratoconjunctival epithelium, VApal eye drops were administered 6 times a day for 7 days. The efficacy of VApal was also compared with that of 0.1% hyaluronic acid eye drops.
The fluorescein staining and rose bengal scores showed a significant decrease compared with the score in the vehicle group at 7 days (P < 0.05) in the 1000 IU/mL VApal group and at both 3 days (P < 0.05) and 7 days (P < 0.01) in the 1500 IU/mL VApal group. Histological examination revealed recovery of the corneal epithelium, and PAS staining disclosed the recovery of mucin-producing lower palpebral conjunctival goblet cells after 7 days in the 1500 IU/mL VApal group compared with the vehicle group. Results from impression cytology showed a significant increase in density of conjunctival goblet cells compared with that in the vehicle group after 7 days in the 1000 IU/mL VApal group and after 3 and 7 days in the 1500 IU/mL VApal group. There were no significant changes in tear flow in either group. Topical application of VApal at 1500 IU/mL showed greater improvement than 0.1% hyaluronic acid in both fluorescein and rose bengal score and in the density of conjunctival goblet cells.
It is suggested that VApal is effective for the improvement of keratoconjunctival epithelial damage associated with tear abnormalities, such as dry eyes.
我们使用泪腺切除的干眼模型兔，研究了棕榈酸视黄酯（VApal）对干眼的疗效。
在角结膜上皮进行碱性损伤后，每天6次给予VApal滴眼液，持续7天。还将VApal的疗效与0.1%透明质酸滴眼液进行了比较。
1000 IU/mL VApal组在第7天、1500 IU/mL VApal组在第3天（P<0.05）和第7天（P<0.01）时，荧光素染色和孟加拉玫瑰红评分与赋形剂组相比均显著降低。组织学检查显示角膜上皮恢复，与赋形剂组相比，1500 IU/mL VApal组在7天后PAS染色显示下睑结膜杯状细胞分泌粘蛋白功能恢复。印迹细胞学结果显示，1000 IU/mL VApal组在第7天、1500 IU/mL VApal组在第3天和第7天后，结膜杯状细胞密度与赋形剂组相比显著增加。两组泪液流量均无显著变化。1500 IU/mL的VApal局部应用在荧光素和孟加拉玫瑰红评分以及结膜杯状细胞密度方面显示出比0.1%透明质酸更大的改善。
提示VApal对改善与干眼等泪液异常相关的角结膜上皮损伤有效。
