College of Health Science, Wuhan Sports University, Wuhan, China.
Medical College, Huainan Union University, Huainan, China.
Am J Sports Med. 2022 Apr;50(5):1358-1368. doi: 10.1177/03635465211073339. Epub 2022 Feb 21.
Mechanical stimulation and platelet-rich plasma (PRP) have been shown to be beneficial for healing of the bone-tendon interface (BTI), but few studies have explored the efficacy of a combination of these applications. We investigated the effect of mechanical stimulation combined with PRP on rotator cuff repair in mice.
Mechanical stimulation combined with PRP can enhance BTI healing in a murine model of rotator cuff repair.
Controlled laboratory study.
A total of 160 C57BL/6 mice were used. Overall, 40 mice were used to prepare PRP, while 120 mice underwent acute supraspinatus tendon (SST) repair. The animals were randomly assigned to 4 groups: control group, mechanical stimulation group, PRP group, and mechanical stimulation combined with PRP group (combination group). At 4 and 8 weeks postoperatively, animals were sacrificed, the eyeballs were removed to collect blood, and the SST-humeral complexes were collected. Histological, biomechanical, immunological, and bone morphometric tests were performed.
Histologically, at 4 and 8 weeks after surgery, the area of the fibrocartilage layer at the BTI in the combination group was larger than in the other groups. The content and distribution of proteoglycans in this layer in the combination group were significantly greater than in the other groups. At 8 weeks postoperatively, trabecular number, and trabecular bone thickness of the subchondral bone area of interest at the BTI of the combination group were greater than those of the other groups, bone volume fraction of the combination group was greater than the control group. On biomechanical testing at 4 and 8 weeks after surgery, the failure load and ultimate strength of the SST-humeral complex in the combination group were higher than in the other groups. Enzyme-linked immunosorbent assay results showed that, at 4 weeks postoperatively, the serum concentrations of transforming growth factor beta 1 and platelet-derived growth factor (PDGF) in the combination group were significantly higher than in the other groups; at 8 weeks, the PDGF-AB concentration in the combination group was higher than in the control and mechanical stimulation groups.
Mechanical stimulation combined with PRP can effectively promote the early stage of healing after a rotator cuff injury.
These findings imply that mechanical stimulation combined with PRP can serve as a potential therapeutic strategy for rotator cuff healing.
机械刺激和富含血小板的血浆（PRP）已被证明对骨-腱结合处（BTI）的愈合有益，但很少有研究探索这些应用的组合效果。我们研究了机械刺激联合 PRP 对小鼠肩袖修复的影响。
机械刺激联合 PRP 可增强小鼠模型肩袖修复中的 BTI 愈合。
对照实验室研究。
使用了 160 只 C57BL/6 小鼠。其中，40 只用于制备 PRP，120 只进行急性冈上肌腱（SST）修复。动物被随机分为 4 组：对照组、机械刺激组、PRP 组和机械刺激联合 PRP 组（联合组）。术后 4 周和 8 周，处死动物，取出眼球采集血液，采集 SST-肱骨复合体。进行组织学、生物力学、免疫学和骨形态计量学测试。
组织学上，术后 4 周和 8 周时，联合组 BTI 纤维软骨层的面积大于其他组。该层中糖胺聚糖的含量和分布在联合组中明显大于其他组。术后 8 周时，联合组 BTI 感兴趣区的骨小梁数量和骨小梁骨厚度大于其他组，联合组的骨体积分数大于对照组。在术后 4 周和 8 周的生物力学测试中，联合组 SST-肱骨复合体的失效负荷和极限强度均高于其他组。酶联免疫吸附试验结果显示，术后 4 周时，联合组血清转化生长因子-β 1 和血小板衍生生长因子（PDGF）浓度明显高于其他组；术后 8 周时，联合组 PDGF-AB 浓度高于对照组和机械刺激组。
机械刺激联合 PRP 可有效促进肩袖损伤后的早期愈合。
这些发现表明，机械刺激联合 PRP 可作为肩袖愈合的潜在治疗策略。
