Peng Yundong, Diao Luyu, Wang Juan, Wang Guanglan, Jia Shaohui, Zheng Cheng
School of Sports Medicine and Rehabilitation, Beijing Sport University, Beijing, China.
College of Sports Medicine, Wuhan Sports University, Wuhan, China.
Orthop J Sports Med. 2024 Feb 22;12(2):23259671231219812. doi: 10.1177/23259671231219812. eCollection 2024 Feb.
Platelet-rich plasma (PRP) has demonstrated beneficial effects on healing of the bone-tendon interface (BTI).
To determine the optimal initiation time for PRP application after rotator cuff repair in an animal model.
Controlled laboratory study.
A total of 136 C57BL/6 mice were included; 40 mice were used to prepare PRP, while 96 mice underwent acute supraspinatus tendon (SST) repair. The animals were randomly divided into 4 groups: a control group and 3 groups in which PRP was injected into the injury interface immediately after surgery, on the 7th postoperative day (PRP-7d), and on the 14th postoperative day. At 4 and 8 weeks postoperatively, the animals were sacrificed, blood was collected by eyeball removal, and samples of the SST-humerus complex were collected. Histological, imaging, immunological, and biomechanical data were compared among the groups using 1-way analysis of variance with the Bonferroni post hoc test.
Histological analysis revealed that the fibrocartilage layer at the BTI was larger in the PRP-7d group compared to the other groups at both 4 and 8 weeks postoperatively. Moreover, the PRP-7d group exhibited improved proteoglycan content and distribution compared to the other groups. Enzyme-linked immunosorbent assay results demonstrated that at 4 weeks postoperatively, higher concentrations of transforming growth factor-β1 and platelet-derived growth factor-BB (PDGF-BB) were seen in the PRP-7d group versus the PRP-14d and control gruops ( < .05), and at 8 weeks postoperatively, the concentration of PDGF-BB was higher in the PRP-7d group versus the control group ( < .05). Biomechanical testing at 4 weeks postoperatively revealed that the failure load and ultimate strength of the SST-humerus complex were superior in the PRP-7d group compared to the other groups ( < .05), at 8 weeks, PRP-7d group was superior to the control group ( < .05). Additionally, at 8 weeks postoperatively, the PRP-7d group exhibited a greater trabecular number and trabecular thickness at the BTI compared to the PRP-14d and control gruops ( < .05).
PRP promoted healing of the BTI after a rotator cuff injury at an early stage.
A PRP injection on the 7th postoperative day demonstrated superior therapeutic effects compared with injections at other time points.
富血小板血浆（PRP）已证明对骨-肌腱界面（BTI）的愈合具有有益作用。
在动物模型中确定肩袖修复术后PRP应用的最佳起始时间。
对照实验室研究。
共纳入136只C57BL/6小鼠；40只小鼠用于制备PRP，96只小鼠接受急性冈上肌腱（SST）修复。动物被随机分为4组：1个对照组和3个PRP注射组，分别在术后立即、术后第7天（PRP-7d）和术后第14天将PRP注入损伤界面。术后4周和8周时，处死动物，通过摘除眼球采集血液，并采集SST-肱骨复合体样本。使用单因素方差分析和Bonferroni事后检验对各组之间的组织学、影像学、免疫学和生物力学数据进行比较。
组织学分析显示，术后4周和8周时，PRP-7d组BTI处的纤维软骨层均大于其他组。此外，与其他组相比，PRP-7d组的蛋白聚糖含量和分布有所改善。酶联免疫吸附测定结果表明，术后4周时，PRP-7d组的转化生长因子-β1和血小板衍生生长因子-BB（PDGF-BB）浓度高于PRP-14d组和对照组（P<0.05）；术后8周时，PRP-7d组的PDGF-BB浓度高于对照组（P<0.05）。术后4周的生物力学测试显示，PRP-7d组SST-肱骨复合体的破坏载荷和极限强度优于其他组（P<0.05）；术后8周时，PRP-7d组优于对照组（P<0.05）。此外，术后8周时，与PRP-14d组和对照组相比，PRP-7d组BTI处的骨小梁数量和骨小梁厚度更大（P<0.05）。
PRP可促进肩袖损伤后早期BTI的愈合。
术后第7天注射PRP与其他时间点注射相比，显示出更好的治疗效果。
