Asaoka Kentaro, Sasaki Kenji, Yagi Jurina, Takahashi Fumiko, Nagano Kenichi, Yamamoto Tetsuji, Sugano Ikuo, Okano Tetsuya, Hasumi Motomitsu, Nakatani Yoshiko, Takano Tomonori, Yamasaki Yukitaka, Ookawa Junko, Kunishima Hiroyuki
Department of Research and Development, Household Products Research, Kao Corporation, Wakayama City, Wakayama prefecture, Japan.
Department of Research and Development, Safety Science Research, Kao Corporation, Haga-gun, Tochigi prefecture, Japan.
J Infect Chemother. 2022 May;28(5):684-689. doi: 10.1016/j.jiac.2022.01.023. Epub 2022 Feb 18.
Staphylococcus aureus colonizes rough regions of the skin of the hand. Healing of S. aureus-mediated wounds is promoted by the application of RNA III inhibiting peptide, which inhibits the production of S. aureus virulence factors, including δ-toxin. Herein, we investigated the level of hand-skin roughness in healthcare professionals after they used an alcohol-based hand rub containing polyoxyethylene lauryl ether (formulation E), which inhibits S. aureus δ-toxin production.
The inhibition rate of S. aureus δ-toxin production by hand rubs, including formulation E, was calculated by quantifying S. aureus δ-toxin concentration in culture medium using high-performance liquid chromatography. Healthcare professionals used formulations E or S (reference alcohol-based hand rub) for 4 weeks. The surface evaluation of the scaliness (SEsc) value was used as an indicator of hand skin roughness. The ΔSEsc value was calculated by subtracting the SEsc value before using the alcohol-based hand rub from the SEsc value 4 weeks after use.
The inhibition rates of S. aureus δ-toxin production by formulations E and S were 43% and 10%, respectively. Formulation E significantly reduced ΔSEsc. The difference in ΔSEsc values after using formulations E and S was significant.
The inhibitory effect on S. aureus δ-toxin production was higher with formulation E than with formulation S. Compared to formulations S, formulation E was effective at reducing scaliness and alleviating hand-skin roughness. Furthermore, the inhibitory effect of formulation E on S. aureus δ-toxin production could be associated with a reduction in scaliness and alleviation of hand-skin roughness.
金黄色葡萄球菌定殖于手部皮肤的粗糙区域。应用RNA III抑制肽可促进由金黄色葡萄球菌介导的伤口愈合，该肽可抑制包括δ-毒素在内的金黄色葡萄球菌毒力因子的产生。在此，我们调查了医护人员使用含聚氧乙烯月桂醚的酒精擦手液（配方E）后手部皮肤粗糙度的水平，该擦手液可抑制金黄色葡萄球菌δ-毒素的产生。
通过高效液相色谱法定量培养基中金黄色葡萄球菌δ-毒素的浓度，计算包括配方E在内的擦手液对金黄色葡萄球菌δ-毒素产生的抑制率。医护人员使用配方E或S（参考酒精擦手液）4周。将鳞屑度（SEsc）值的表面评估用作手部皮肤粗糙度的指标。ΔSEsc值通过使用酒精擦手液4周后的SEsc值减去使用前的SEsc值来计算。
配方E和S对金黄色葡萄球菌δ-毒素产生的抑制率分别为43%和10%。配方E显著降低了ΔSEsc。使用配方E和S后ΔSEsc值的差异显著。
配方E对金黄色葡萄球菌δ-毒素产生的抑制作用高于配方S。与配方S相比，配方E在减少鳞屑和减轻手部皮肤粗糙度方面有效。此外，配方E对金黄色葡萄球菌δ-毒素产生的抑制作用可能与鳞屑减少和手部皮肤粗糙度减轻有关。
