Comparison of the chemical composition and antioxidant stress ability of polysaccharides from under different drying methods.

fruiting body-derived polysaccharides (AAPs) were dried using different drying procedures, including hot air-, far infrared-, freeze-, and microwave-drying. The influences of different drying procedures on the chemical compositions and antioxidant activity and of AAPs were investigated. The results indicated that freeze-dried AAPs (AAPs-F) possessed the highest uronic acid content (33.53%) and the lowest molecular weight (406.77 kDa). Moreover, AAPs-F exhibited the most potent antioxidant abilities , including ABTS and DPPH˙ scavenging abilities, ferric reducing power, and metal ion chelating capacity. Besides, AAPs-F could significantly prolong the lifespan of wild-type under oxidative stress induced by HO and methyl viologen ( < 0.05) and upregulate the mRNA expression levels of (>2.7 fold), (>9.2 fold), (>4.5 fold) and (>1.9 fold), and play a significant role in protecting against apoptosis ( < 0.05). Hence, freeze-drying was determined as the preferred procedure for obtaining high-quality AAPs.