Al-Madhagy Somaia A, Gad Sameh S, Mostafa Eman S, Angeloni Simone, Saad Muhammed A, Sabry Omar M, Caprioli Giovani, El-Hawary Seham S
Department of Pharmacognosy, College of Pharmacy, Cairo University, Cairo, Egypt.
Department of Pharmacognosy, Faculty of Pharmacy, Sana'a University, Sana'a, Yemen.
Nat Prod Res. 2022 Dec;36(23):6075-6080. doi: 10.1080/14786419.2022.2044811. Epub 2022 Feb 22.
Fifteen compounds belong to phenolic acids, derivatives of phenolic acids, iridoids, xanthones and flavonoids were characterized in the methanolic extract of leaves using HPLC-MS/MS. Extract has been also investigated for its MAO-B inhibitory activity, antioxidant activity, total phenolic and total content. The extract exhibited interesting MAO-B inhibitory activity (IC50; 2.24 ± 0.08) compared to the reference compound selegiline (0.55 ± 0.02 µg/mL). It also showed a potent antioxidant activity proven in both DPPH and ORAC assay methods. The extract showed an IC50 of 3.64 ± 1.22 µg/mL in the DPPH test which was significantly lower than that of the standard ascorbic acid which attained an IC50 of 18.3 ± 1.41 µg/mL. Moreover, in the oxygen radical absorbance capacity assay (ORAC) the extract showed a decline in the IC50 to 3.48 ± 1.16 µg/mL as compared to the standard Trolox which exhibited an IC50 of 27.0 ± 13.41.
采用高效液相色谱-串联质谱法（HPLC-MS/MS）对叶片甲醇提取物中的15种化合物进行了表征，这些化合物属于酚酸、酚酸衍生物、环烯醚萜、呫吨酮和黄酮类。还对该提取物的单胺氧化酶B（MAO-B）抑制活性、抗氧化活性、总酚含量和总含量进行了研究。与参比化合物司来吉兰（0.55±0.02μg/mL）相比，该提取物表现出有趣的MAO-B抑制活性（半数抑制浓度IC50；2.24±0.08）。在二苯基苦味酰基自由基（DPPH）和氧自由基吸收能力（ORAC）测定方法中，该提取物均显示出较强的抗氧化活性。在DPPH试验中，该提取物的IC50为3.64±1.22μg/mL，显著低于标准抗坏血酸，后者的IC50为18.3±1.41μg/mL。此外，在ORAC测定中，与标准曲克芦丁（IC50为27.0±13.41）相比，该提取物的IC50降至3.48±1.16μg/mL。
