Shigyo Y, Mori T, Kano K
Immunol Invest. 1986 Mar;15(1):25-33. doi: 10.3109/08820138609042016.
By means of indirect immunofluorescence (IFF) tests with rabbit antiserum to Forssman (F) glycolipid, expression of F antigen was investigated on 16 rat tumor cell lines; 14 chemical carcinogen-induced tumors, one yolk sac-derived (AT-1) tumor and another spontaneous tumor. Four lines of the carcinogen-induced tumors gave weakly positive reactions in the IIF tests and the AT-1 cells a strongly positive reaction. Flow fluorocytometric analyses on the AT-1 cell line revealed that some of the major and the vast majority of the minor population of the AT-1 express F antigen. Specificity of the F antigen demonstrated on AT-1 cells was determined by absorption experiments, in which absorption of the F antiserum with guinea pig kidney sediment, sheep red blood cells and F-liposome but not bovine red blood cells or G-liposome abolished the reaction. Results of this study together with those of our previous studies demonstrated that F antigen appears as a result of malignant transformation of F negative rat cells induced by chemical carcinogens, apparently "distorted differentiation" and the viral oncogenes.
利用兔抗福斯曼（F）糖脂抗血清进行间接免疫荧光（IFF）试验，研究了16种大鼠肿瘤细胞系中F抗原的表达情况；其中包括14种化学致癌物诱导的肿瘤、1种卵黄囊来源的（AT - 1）肿瘤以及另一种自发肿瘤。在IIF试验中，4种致癌物诱导的肿瘤细胞系呈弱阳性反应，而AT - 1细胞呈强阳性反应。对AT - 1细胞系进行流式荧光细胞术分析表明，AT - 1细胞中的一些主要细胞群体以及绝大多数次要细胞群体表达F抗原。通过吸收实验确定了AT - 1细胞上显示的F抗原的特异性，即用豚鼠肾沉淀物、绵羊红细胞和F - 脂质体吸收F抗血清，但用牛红细胞或G - 脂质体吸收则不能消除反应。本研究结果与我们之前的研究结果表明，F抗原是化学致癌物诱导的F阴性大鼠细胞恶性转化的结果，显然是“分化异常”和病毒癌基因导致的。
