Mori T, Sudo T, Kano K
J Natl Cancer Inst. 1983 May;70(5):811-8.
By means of indirect immunofluorescence (IIF) tests with rabbit antiserum against Forssman (F) antigen, F-antigen was demonstrated on 5 of 7 cultured cell lines originating from various cancer tissues. Results of cytotoxicity tests with normal human sera with F-antibodies confirmed the presence of F-antigen on these "IIF-positive" cell lines. In contrast, F-antigen could not be detected under the same experimental conditions on B-cell lines established from lymphoma or leukemia patients or on lymphoblastoid cell lines established from normal individuals. The expression of F-antigen was also studied on transformed rat cell lines derived from an F-negative normal cell line, 3y1. Transformation of the 3y1 line by whole DNA or by the EcoRI-C fragment (0-16 map unit) but not by the Accl-H fragment (0-4.7 map unit) of adenovirus type 12 resulted in expression of F-antigen on the cell surface. The transformed cells with F-antigen showed an in vitro growth pattern characteristic of that seen in malignant cells; this observation indicates that F-antigen expression is closely associated with malignant transformation of the cells.
通过使用兔抗福斯曼（F）抗原抗血清进行间接免疫荧光（IIF）试验，在源自各种癌组织的7种培养细胞系中的5种上证实了F抗原的存在。用含有F抗体的正常人血清进行细胞毒性试验的结果证实了这些“IIF阳性”细胞系上存在F抗原。相比之下，在相同实验条件下，从淋巴瘤或白血病患者建立的B细胞系或从正常个体建立的淋巴母细胞系上未检测到F抗原。还对源自F阴性正常细胞系3y1的转化大鼠细胞系上F抗原的表达进行了研究。用12型腺病毒的全DNA或EcoRI-C片段（0-16图谱单位）而非AccI-H片段（0-4.7图谱单位）对3y1细胞系进行转化，导致细胞表面表达F抗原。具有F抗原的转化细胞呈现出恶性细胞特有的体外生长模式；这一观察结果表明F抗原表达与细胞的恶性转化密切相关。
