Mori T, Mori E, Sudo T, Kano K
J Natl Cancer Inst. 1984 Nov;73(5):1179-86.
Monoclonal Forssman (F) antibodies of the IgM class were obtained by hybridization of spleen cells from an immunized F344 rat with murine myeloma cells. By means of the indirect immunofluorescence test with monoclonal F-antibodies, F-antigen was demonstrated on rat cell lines derived from a normal cell line that was transformed by transfection with whole adenovirus type 12 DNA or a fragment (E1a + E1b) of adenovirus type 12 DNA. These transformed cells were shown to shed the F-antigen into the culture supernatant, depending on their degree of malignancy. The F-antigen was demonstrated in a glycoprotein, but not in a glycolipid fraction of the supernatants. The glycoprotein purified by affinity chromatography was subjected to gel electrophoresis and subsequent Western blotting. The F-active molecules were identified as three distinct bands of approximately 130,000, 60,000, and 27,000.
通过将免疫后的F344大鼠的脾细胞与鼠骨髓瘤细胞杂交，获得了IgM类单克隆福斯曼（F）抗体。通过使用单克隆F抗体进行间接免疫荧光试验，在源自正常细胞系的大鼠细胞系上证实了F抗原，该正常细胞系通过用完整的12型腺病毒DNA或12型腺病毒DNA片段（E1a + E1b）转染而转化。这些转化细胞显示出根据其恶性程度将F抗原释放到培养上清液中。在糖蛋白中证实了F抗原，但在上清液的糖脂部分中未证实。通过亲和层析纯化的糖蛋白进行凝胶电泳和随后的蛋白质印迹分析。F活性分子被鉴定为三条不同的条带，大小约为130,000、60,000和27,000。
