Mori T, Mori E, Shigyo Y, Sudo T, Kano K
J Natl Cancer Inst. 1986 Jul;77(1):115-20.
Immunobiological significance of Forssman (F) glycoprotein expressed on rat tumor cells derived from transfection of a fibroblast line with whole DNA (WY-3); EcoRI-C fragment, 0-16.5 map units (CY-1); and Accl-H fragment, 0-4.7 map units (HY-1) of adenovirus 12 was investigated. Culturing of F-positive WY-3 and CY-1 cells, but not F-negative HY-1 cells, with monoclonal rat F-antibody resulted in the blocking of their cell-cell adhesion and attachment to plastic surface and inhibition of their growth. Immunization of WY-3 or CY-1 tumor-bearing F344 rats with sheep red blood cells or purified F-antigen in adjuvants brought regression of the tumor in 13 of 19 rats. No such antitumor effect was observed in F-negative HY-1 tumor-bearing rats upon immunization with the F-antigens. Results of this study indicated that the membrane glycoprotein with F-epitope may play a role in lodgement of the tumor cells in vitro and serve as an in vivo target of specific immune effectors.
研究了用完整DNA（WY - 3）转染成纤维细胞系、腺病毒12的EcoRI - C片段（0 - 16.5图谱单位，CY - 1）以及AccI - H片段（0 - 4.7图谱单位，HY - 1）后在大鼠肿瘤细胞上表达的福斯曼（F）糖蛋白的免疫生物学意义。用单克隆大鼠F抗体培养F阳性的WY - 3和CY - 1细胞（而非F阴性的HY - 1细胞），导致它们的细胞间黏附以及与塑料表面的附着被阻断，并抑制其生长。用佐剂中的绵羊红细胞或纯化的F抗原免疫携带WY - 3或CY - 1肿瘤的F344大鼠，19只大鼠中有13只肿瘤出现消退。在用F抗原免疫时，未在携带F阴性HY - 1肿瘤的大鼠中观察到这种抗肿瘤作用。本研究结果表明，具有F表位的膜糖蛋白可能在体外肿瘤细胞的着床中起作用，并作为体内特异性免疫效应物的靶点。
