Coutelier J P, Van Roost E, Lambotte P, Van Snick J
J Gen Virol. 1986 Jun;67 ( Pt 6):1099-108. doi: 10.1099/0022-1317-67-6-1099.
Mice infected with lactate dehydrogenase-elevating virus (LDV) were found to produce high titres of IgG anti-LDV antibodies that remained elevated for more than 1 year. This response, which was T-dependent, showed a striking preponderance of IgG2a with, from one strain to another, variable proportions of IgG2b and IgG3 but always very little IgG1. The binding of these antibodies to viral protein blots showed a major reaction with VP3, the heterogeneous glycosylated material of the viral envelope. A minor reaction was also noted with VP1, the nucleocapsid protein, but no antibodies were detected against VP2, the non-glycosylated envelope protein of LDV. A similar preponderance of anti-VP3 antibodies was also observed in a large set of anti-LDV hybridomas. Analysis of VP3 with monoclonal antibodies suggested that, despite its heterogeneity, this material has a common polypeptide moiety that apparently carries two major epitopes.
感染乳酸脱氢酶升高病毒（LDV）的小鼠被发现产生高滴度的抗LDV IgG抗体，这些抗体在1年多的时间里一直保持在高水平。这种T细胞依赖性反应显示，IgG2a占显著优势，不同品系的小鼠中IgG2b和IgG3的比例各不相同，但IgG1的比例始终很低。这些抗体与病毒蛋白印迹的结合显示，与VP3有主要反应，VP3是病毒包膜的异质性糖基化物质。与核衣壳蛋白VP1也有轻微反应，但未检测到针对LDV非糖基化包膜蛋白VP2的抗体。在大量抗LDV杂交瘤中也观察到类似的抗VP3抗体优势。用单克隆抗体对VP3进行分析表明，尽管VP3具有异质性，但这种物质有一个共同的多肽部分，该部分显然带有两个主要表位。
