• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于显微镜的成体、新生和 hiPSC 心肌细胞细胞收缩性分析检测。

Microscopy-based cellular contractility assay for adult, neonatal, and hiPSC cardiomyocytes.

机构信息

Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil.

Departamento de Física, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil.

出版信息

STAR Protoc. 2022 Feb 11;3(1):101144. doi: 10.1016/j.xpro.2022.101144. eCollection 2022 Mar 18.

DOI:10.1016/j.xpro.2022.101144
PMID:35199028
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8850799/
Abstract

This protocol provides instructions to acquire high-quality cellular contractility data from adult, neonatal, and human induced pluripotent stem cell-derived cardiomyocytes. Contractility parameters are key to unravel mechanisms underlying cardiac pathologies, yet difficulties in acquiring data can compromise measurement accuracy and reproducibility. We provide optimized steps for microscope and camera setup, as well as cellular selection criteria for different cardiomyocyte cell types, aiming to obtain robust and reliable data. Moreover, we use CONTRACTIONWAVE software to analyze and show the optimized results. For complete details on the use and execution of this profile, please refer to Scalzo et al. (2021).

摘要

本方案提供了从成年、新生和人诱导多能干细胞衍生的心肌细胞中获取高质量细胞收缩性数据的操作说明。收缩性参数是揭示心脏病理机制的关键，但数据获取的困难可能会影响测量的准确性和重现性。我们提供了显微镜和相机设置的优化步骤，以及针对不同心肌细胞类型的细胞选择标准，旨在获得稳健可靠的数据。此外，我们还使用 CONTRACTIONWAVE 软件进行分析并展示优化结果。有关该方案使用和执行的详细信息，请参阅 Scalzo 等人（2021 年）的文献。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/8b7383d204f1/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/6ea97bcd97de/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/50a6acbf191f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/af8b7a626ded/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/dda15c005f87/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/41c197f66682/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/35b911ac0d08/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/221eba988d1c/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/ad671652fe06/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/0792132c91e1/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/95bcacfd8508/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/cf05d1628102/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/8b7383d204f1/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/6ea97bcd97de/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/50a6acbf191f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/af8b7a626ded/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/dda15c005f87/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/41c197f66682/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/35b911ac0d08/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/221eba988d1c/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/ad671652fe06/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/0792132c91e1/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/95bcacfd8508/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/cf05d1628102/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7b43/8850799/8b7383d204f1/gr11.jpg

相似文献

1
Microscopy-based cellular contractility assay for adult, neonatal, and hiPSC cardiomyocytes.基于显微镜的成体、新生和 hiPSC 心肌细胞细胞收缩性分析检测。
STAR Protoc. 2022 Feb 11;3(1):101144. doi: 10.1016/j.xpro.2022.101144. eCollection 2022 Mar 18.
2
Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes.人诱导多能干细胞衍生心肌细胞收缩、钙和动作电位测量方案。
STAR Protoc. 2021 Oct 8;2(4):100859. doi: 10.1016/j.xpro.2021.100859. eCollection 2021 Dec 17.
3
Dense optical flow software to quantify cellular contractility.用于量化细胞收缩力的密集光流软件。
Cell Rep Methods. 2021 Jul 7;1(4):100044. doi: 10.1016/j.crmeth.2021.100044. eCollection 2021 Aug 23.
4
Speckle-Tracking Strain Analysis for Mapping Spatiotemporal Contractility of Induced Pluripotent Stem Cell (iPSC)-Derived Cardiomyocytes.斑点追踪应变分析用于绘制诱导多能干细胞（iPSC）衍生心肌细胞的时空收缩性。
Curr Protoc. 2023 Sep;3(9):e889. doi: 10.1002/cpz1.889.
5
Automated Dynamic Clamp for Simulation of I in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Real Time Using Patchliner Dynamite.使用Patchliner Dynamite实时模拟人诱导多能干细胞衍生心肌细胞中I的自动动态钳制
Curr Protoc Pharmacol. 2020 Mar;88(1):e70. doi: 10.1002/cpph.70.
6
Human-induced pluripotent stem cell-derived cardiomyocytes from cardiac progenitor cells: effects of selective ion channel blockade.源自心脏祖细胞的人诱导多能干细胞衍生心肌细胞：选择性离子通道阻断的作用
Europace. 2016 Dec;18(suppl 4):iv67-iv76. doi: 10.1093/europace/euw352.
7
Acute effects of cardiac contractility modulation on human induced pluripotent stem cell-derived cardiomyocytes.心肌收缩调节对人诱导多能干细胞衍生心肌细胞的急性影响。
Physiol Rep. 2021 Nov;9(21):e15085. doi: 10.14814/phy2.15085.
8
A simple protocol to produce mature human-induced pluripotent stem cell-derived cardiomyocytes.一种简单的方案可生成成熟的人诱导多能干细胞衍生的心肌细胞。
STAR Protoc. 2021 Oct 25;2(4):100912. doi: 10.1016/j.xpro.2021.100912. eCollection 2021 Dec 17.
9
Same-Single-Cell Analysis of Pacemaker-Specific Markers in Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Subtypes Classified by Electrophysiology.对通过电生理学分类的人诱导多能干细胞衍生心肌细胞亚型中起搏器特异性标志物的单细胞分析
Stem Cells. 2016 Nov;34(11):2670-2680. doi: 10.1002/stem.2466. Epub 2016 Jul 29.
10
Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of fatty acid synthesis.通过抑制脂肪酸合成从人多能干细胞中纯化心肌细胞和神经元。
STAR Protoc. 2022 Apr 28;3(2):101360. doi: 10.1016/j.xpro.2022.101360. eCollection 2022 Jun 17.

引用本文的文献

1
Highly Durable, Stretchable Multielectrode Array for Electro-mechanical Co-stimulation of Cells.用于细胞机电联合刺激的高耐用性、可拉伸多电极阵列
Biomater Res. 2024 Jun 30;28:0030. doi: 10.34133/bmr.0030. eCollection 2024.
2
Empagliflozin reduces arrhythmogenic effects in rat neonatal and human iPSC-derived cardiomyocytes and improves cytosolic calcium handling at least partially independent of NHE1.恩格列净可减少大鼠新生期和人诱导多能干细胞衍生的心肌细胞的致心律失常作用，并至少部分独立于钠氢交换蛋白 1 改善细胞溶质钙处理。
Sci Rep. 2023 May 29;13(1):8689. doi: 10.1038/s41598-023-35944-5.
3
Protocol to measure calcium spikes in cardiomyocytes obtained from human pluripotent stem cells using a ready-to-use media.

本文引用的文献

1
Dense optical flow software to quantify cellular contractility.用于量化细胞收缩力的密集光流软件。
Cell Rep Methods. 2021 Jul 7;1(4):100044. doi: 10.1016/j.crmeth.2021.100044. eCollection 2021 Aug 23.
2
Long-term single-cell passaging of human iPSC fully supports pluripotency and high-efficient trilineage differentiation capacity.人诱导多能干细胞的长期单细胞传代充分维持了多能性和高效的三系分化能力。
SAGE Open Med. 2020 Oct 22;8:2050312120966456. doi: 10.1177/2050312120966456. eCollection 2020.
3
Protocol for Isolation of Viable Adult Rat Cardiomyocytes with High Yield.
使用即用型培养基测量从人多能干细胞获得的心肌细胞中钙尖峰的方案。
STAR Protoc. 2023 Apr 13;4(2):102252. doi: 10.1016/j.xpro.2023.102252.
4
The MyoPulser field stimulator, a do it yourself programmable electronic pacemaker for contracting cells and tissues.MyoPulser 场刺激器，一种用于收缩细胞和组织的 DIY 可编程电子起搏器。
Sci Rep. 2023 Feb 11;13(1):2461. doi: 10.1038/s41598-023-29145-3.
高产量分离成年大鼠心肌细胞的方案。
STAR Protoc. 2020 Jun 9;1(2):100045. doi: 10.1016/j.xpro.2020.100045. eCollection 2020 Sep 18.
4
The "MYOCYTER" - Convert cellular and cardiac contractions into numbers with ImageJ.“MYOCYTER”- 用 ImageJ 将细胞和心脏收缩转化为数字。
Sci Rep. 2019 Oct 22;9(1):15112. doi: 10.1038/s41598-019-51676-x.
5
Gene Therapy for Catecholaminergic Polymorphic Ventricular Tachycardia by Inhibition of Ca/Calmodulin-Dependent Kinase II.钙/钙调蛋白依赖性激酶 II 抑制的儿茶酚胺多形性室性心动过速的基因治疗。
Circulation. 2019 Jul 30;140(5):405-419. doi: 10.1161/CIRCULATIONAHA.118.038514. Epub 2019 Jun 3.
6
MUSCLEMOTION: A Versatile Open Software Tool to Quantify Cardiomyocyte and Cardiac Muscle Contraction In Vitro and In Vivo.肌肉运动：一种通用的开源软件工具，用于量化体外和体内心肌细胞和心肌收缩。
Circ Res. 2018 Feb 2;122(3):e5-e16. doi: 10.1161/CIRCRESAHA.117.312067. Epub 2017 Dec 27.
7
Electrophysiological Properties and Viability of Neonatal Rat Ventricular Myocyte Cultures with Inducible ChR2 Expression.可诱导表达 ChR2 的新生大鼠心室肌细胞培养物的电生理特性和活力。
Sci Rep. 2017 May 8;7(1):1531. doi: 10.1038/s41598-017-01723-2.
8
SarcOptiM for ImageJ: high-frequency online sarcomere length computing on stimulated cardiomyocytes.用于ImageJ的SarcOptiM：对受刺激的心肌细胞进行高频在线肌节长度计算
Am J Physiol Cell Physiol. 2016 Aug 1;311(2):C277-83. doi: 10.1152/ajpcell.00094.2016. Epub 2016 Jun 22.
9
Sarcomere length dependent effects on the interaction between cTnC and cTnI in skinned papillary muscle strips.肌节长度对去表皮乳头肌条中肌钙蛋白C（cTnC）与肌钙蛋白I（cTnI）相互作用的依赖性影响。
Arch Biochem Biophys. 2016 Jul 1;601:69-79. doi: 10.1016/j.abb.2016.02.030. Epub 2016 Mar 2.
10
Maturation status of sarcomere structure and function in human iPSC-derived cardiac myocytes.人诱导多能干细胞衍生心肌细胞中肌节结构与功能的成熟状态
Biochim Biophys Acta. 2016 Jul;1863(7 Pt B):1829-38. doi: 10.1016/j.bbamcr.2015.11.005. Epub 2015 Nov 11.