Kim A Ri, Shrivastava Sajal, Lee Han-Byeol, Lee Nae-Eung
Department of Nano Science and Technology, Sungkyunkwan University, Suwon, Gyeonggi-do 16419, Republic of Korea.
Department of Radiology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Biomater Res. 2024 Jun 30;28:0030. doi: 10.34133/bmr.0030. eCollection 2024.
Electro-mechanical co-stimulation of cells can be a useful cue for tissue engineering. However, reliable co-stimulation platforms still have limitations due to low durability of the components and difficulty in optimizing the stimulation parameters. Although various electro-mechanical co-simulation systems have been explored, integrating materials and components with high durability is still limited. To tackle this problem, we designed an electro-mechanical co-stimulation system that facilitates uniaxial cyclic stretching, electrical stimulation, and optical monitoring. This system utilizes a robust and autoclavable stretchable multielectrode array housed within a compact mini-incubator. To illustrate its effectiveness, we conducted experiments that highlighted how electro-mechanical co-stimulation using this system can enhance the maturation of cardiomyocytes derived from human induced pluripotent stem cells. The results showed great potential of our co-stimulation platform as an effective tool for tissue engineering.
细胞的机电联合刺激对于组织工程而言可能是一种有用的信号。然而，由于组件耐久性低以及刺激参数优化困难，可靠的联合刺激平台仍存在局限性。尽管已经探索了各种机电联合模拟系统，但将具有高耐久性的材料和组件集成起来的情况仍然有限。为了解决这个问题，我们设计了一种机电联合刺激系统，该系统便于进行单轴循环拉伸、电刺激和光学监测。该系统利用了一个坚固且可高压灭菌的可拉伸多电极阵列，该阵列置于一个紧凑的小型培养箱内。为了说明其有效性，我们进行了实验，突出展示了使用该系统的机电联合刺激如何能够促进源自人类诱导多能干细胞的心肌细胞的成熟。结果表明，我们的联合刺激平台作为组织工程的有效工具具有巨大潜力。
