Ghit Amr, Deeb Hany El
Department of Biotechnology, Institute of Graduate Studies and Research (IGSR), Alexandria University, Alexandria, Egypt.
Department of Biology and Biotechnology, University of Pavia, Pavia, Italy.
J Mol Neurosci. 2022 May;72(5):1133-1140. doi: 10.1007/s12031-022-01984-1. Epub 2022 Feb 23.
Parkinson's disease (PD) is one of the most common long-term degenerative disorders of the CNS that primarily affects the human locomotor system. Owing to the heterogeneity of PD etiology and the lack of appropriate diagnostic tests, blood-based biomarkers became the most promising method for diagnosing PD. Even though various biomarkers for PD have been found, their specificity and sensitivity are not optimum when used alone. Therefore, the aim of this study was directed to evaluate changes in a group of sensitive blood-based biomarkers in the same PD patients compared to healthy individuals. Serum samples were collected from 20 PD patients and 15 age-matched healthy controls. We analyzed serum levels of cytokines (IL10, IL12, and TNF-α), α-synuclein proteins, miRNAs (miR-214, miR-221, and miR-141), and antioxidants (UA, PON1, ARE). Our results showed an increase in sera levels of cytokines in PD patients as well as a positive correlation among them. Also, we found a significant increase in sera levels of α-synuclein protein associated with a decrease in miR-214 which regulates its gene expression. Lastly, we observed a decrease in sera levels of miR-221, miR-141, UA, PON1, and ARE, which have a prominent role against oxidative stress. Because of the many etiologies of PD, a single measure is unlikely to become a useful biomarker. Therefore, to correctly predict disease state and progression, a mix of noninvasive biomarkers is required. Although considerable work has to be done, this study sheds light on the role of certain biomarkers in the diagnosis of PD.
帕金森病（PD）是中枢神经系统最常见的长期退行性疾病之一，主要影响人体运动系统。由于帕金森病病因的异质性以及缺乏合适的诊断测试，基于血液的生物标志物成为诊断帕金森病最有前景的方法。尽管已经发现了多种帕金森病的生物标志物，但单独使用时它们的特异性和敏感性并不理想。因此，本研究的目的是评估同一组帕金森病患者与健康个体相比，一组敏感的基于血液的生物标志物的变化。收集了20例帕金森病患者和15例年龄匹配的健康对照的血清样本。我们分析了细胞因子（IL10、IL12和TNF-α）、α-突触核蛋白、微小RNA（miR-214、miR-221和miR-141）以及抗氧化剂（尿酸、对氧磷酶1、芳烃受体）的血清水平。我们的结果显示，帕金森病患者血清中细胞因子水平升高，且它们之间呈正相关。此外，我们发现α-突触核蛋白血清水平显著升高，同时调节其基因表达的miR-214水平降低。最后，我们观察到miR-221、miR-141、尿酸、对氧磷酶1和芳烃受体的血清水平降低，它们在对抗氧化应激方面发挥着重要作用。由于帕金森病病因众多，单一指标不太可能成为有用的生物标志物。因此，为了正确预测疾病状态和进展，需要多种非侵入性生物标志物的组合。尽管还有大量工作要做，但本研究揭示了某些生物标志物在帕金森病诊断中的作用。
