Queensland Micro-Nanotechnology Centre, Griffith University, Nathan, QLD 4111, Australia.
Biorheology Research Laboratory, Menzies Health Institute Queensland, Griffith University, Gold Coast, QLD 4222, Australia.
Biosensors (Basel). 2022 Feb 14;12(2):120. doi: 10.3390/bios12020120.
Plasma extraction from blood is essential for diagnosis of many diseases. The critical process of plasma extraction requires removal of blood cells from whole blood. Fluid viscoelasticity promotes cell migration towards the central axis of flow due to differences in normal stress and physical properties of cells. We investigated the effects of altering fluid viscoelasticity on blood plasma extraction in a serpentine microchannel. Poly (ethylene oxide) (PEO) was dissolved into blood to increase its viscoelasticity. The influences of PEO concentration, blood dilution, and flow rate on the performance of cell focusing were examined. We found that focusing performance can be significantly enhanced by adding PEO into blood. The optimal PEO concentration ranged from 100 to 200 ppm with respect to effective blood cell focusing. An optimal flow rate from 1 to 15 µL/min was determined, at least for our experimental setup. Given less than 1% haemolysis was detected at the outlets in all experimental combinations, the proposed microfluidic methodology appears suitable for applications sensitive to haemocompatibility.
从血液中提取血浆对于许多疾病的诊断至关重要。血浆提取的关键过程需要将血细胞从全血中去除。由于正常应力和细胞物理性质的差异，流体粘弹性促使细胞向流动的中心轴迁移。我们研究了改变流体粘弹性对蛇形微通道中血液血浆提取的影响。将聚氧化乙烯（PEO）溶解在血液中以增加其粘弹性。研究了 PEO 浓度、血液稀释度和流速对细胞聚焦性能的影响。我们发现，通过向血液中添加 PEO 可以显著增强聚焦性能。对于有效血细胞聚焦，最佳的 PEO 浓度范围为 100 至 200ppm。确定了 1 至 15μL/min 的最佳流速，至少对于我们的实验设置是这样。由于在所有实验组合中在出口处检测到的溶血率小于 1%，因此所提出的微流控方法似乎适用于对血液相容性敏感的应用。
