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过表达黑色素瘤来源的细胞外囊泡的抗转移功能。

Anti-Metastatic Function of Extracellular Vesicles Derived from -Overexpressing Melanoma.

机构信息

Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.

Department of Industrial Technology and Innovation, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.

出版信息

Curr Oncol. 2022 Feb 11;29(2):1029-1046. doi: 10.3390/curroncol29020088.

DOI:10.3390/curroncol29020088
PMID:35200587
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8870779/
Abstract

A metastatic melanoma cell line B16-F10 (F10) was modified to a more undifferentiated state by overexpression. The produced cell line F10 showed a higher metastatic potential than F10. Instead of whole cells, the extracellular vesicles (EVs) therefrom were investigated about their possible role as an autovaccine against metastasis. EVs from F10 cells (F10-EVs) could suppress the metastasis, contrasting the EVs from less metastatic F10 cells (F10-EVs) enhanced metastasis. The involvement of TGF-β1 in the role of F10-EVs was analyzed, as TGF-β1 was a secretory cytokine being affected most intensively by overexpression. It was suggested to be crucial that the TGF-β1 concentration in F10-EVs should be as low as 1.6 pg/μg for its metastasis-suppressive role. In response to F10-EVs, immunoreaction was observed in liver, indicating the specific decrease in the number of tumor-promotive CD163-positive macrophages. These indicate a possibility of F10-EVs as a novel autovaccine candidate against melanoma metastasis.

摘要

一个转移性黑色素瘤细胞系 B16-F10（F10）通过过度表达被修饰成更未分化的状态。产生的细胞系 F10 显示出比 F10 更高的转移潜力。研究了来自该细胞系的细胞外囊泡（EVs），以探讨其作为针对转移的自体疫苗的可能作用。来自 F10 细胞的 EVs（F10-EVs）可以抑制转移，而来自转移能力较低的 F10 细胞的 EVs（F10-EVs）增强了转移。分析了 TGF-β1 在 F10-EVs 作用中的参与，因为 TGF-β1 是一种分泌细胞因子，受过度表达的影响最强烈。建议 F10-EVs 中的 TGF-β1 浓度应低至 1.6 pg/μg，以发挥其抑制转移的作用。对 F10-EVs 的反应在肝脏中观察到免疫反应，表明促进肿瘤的 CD163 阳性巨噬细胞数量特异性减少。这些表明 F10-EVs 作为一种新型针对黑色素瘤转移的自体疫苗候选物具有可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d66/8870779/77412ec2d949/curroncol-29-00088-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d66/8870779/826d92a68bc5/curroncol-29-00088-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d66/8870779/ea23673acbd9/curroncol-29-00088-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d66/8870779/7748b796a7e2/curroncol-29-00088-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d66/8870779/e89258853174/curroncol-29-00088-g004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d66/8870779/77412ec2d949/curroncol-29-00088-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d66/8870779/826d92a68bc5/curroncol-29-00088-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d66/8870779/ea23673acbd9/curroncol-29-00088-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d66/8870779/7748b796a7e2/curroncol-29-00088-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d66/8870779/e89258853174/curroncol-29-00088-g004a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d66/8870779/77412ec2d949/curroncol-29-00088-g006.jpg

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2
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Mol Cell Biochem. 2021 Jul;476(7):2651-2661. doi: 10.1007/s11010-021-04110-8. Epub 2021 Mar 4.
3
Trends in Research on Exosomes in Cancer Progression and Anticancer Therapy.癌症进展与抗癌治疗中细胞外囊泡的研究趋势
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Int J Mol Sci. 2023 Dec 6;24(24):17206. doi: 10.3390/ijms242417206.
4
Extracellular Vesicles-Based Cell-Cell Communication in Melanoma: New Perspectives in Diagnostics and Therapy.基于细胞外囊泡的黑色素瘤细胞间通讯：诊断和治疗的新视角。
Int J Mol Sci. 2023 Jan 4;24(2):965. doi: 10.3390/ijms24020965.
5
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6
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Exp Anim. 2023 May 17;72(2):183-192. doi: 10.1538/expanim.22-0078. Epub 2022 Oct 27.
Cancers (Basel). 2021 Jan 17;13(2):326. doi: 10.3390/cancers13020326.
4
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5
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