D. John Faulkner Centre for Marine Biodiscovery and Biomedicine, P.O. Box 4, Kinango 80405, Kenya.
Department of Pure and Applied Sciences, Technical University of Mombasa, P.O. Box 90420, Mombasa 80100, Kenya.
Mar Drugs. 2022 Feb 7;20(2):128. doi: 10.3390/md20020128.
Epibiotic bacteria associated with the filamentous marine cyanobacterium were explored as a novel source of antibiotics and to establish whether they can produce cyclodepsipeptides on their own. Here, we report the isolation of micrococcin P1 () (CHNOS; obs. / 1144.21930/572.60381) and micrococcin P2 () (CHNOS; obs. / 1142.20446/571.60370) from a strain of isolated from ' filaments. Interestingly, most bacteria isolated from ' filaments were found to be human pathogens. Stalked diatoms on the filaments suggested a possible terrestrial origin of some epibionts. CuSO·5HO assisted differential genomic DNA isolation and phylogenetic analysis showed that a Kenyan strain of differed from strain CCAP 1446/4 and clones. Organic extracts of the epibiotic bacteria and did not produce cyclodepsipeptides. Further characterization of 24 Firmicutes strains from identified extracts of as most active. Our results showed that the genetic basis for synthesizing micrococcin P1 (), discovered in ATCC 14579, is species/strain-dependent and this reinforces the need for molecular identification of species worldwide and their epibionts. These findings indicate that -associated bacteria are an overlooked source of antimicrobial compounds.
与丝状海洋蓝细菌相关的附生细菌被探索作为抗生素的新来源，并确定它们是否能够自行产生环二肽。在这里，我们报告了从一株分离的微球菌 P1()(CHNOS；观察值/1144.21930/572.60381)和微球菌 P2()(CHNOS；观察值/1142.20446/571.60370)的分离，该菌株来自“”丝状藻。有趣的是，从“”丝状藻上分离出的大多数细菌被发现是人类病原体。丝状藻上的有梗硅藻表明一些附生物可能来自陆地。CuSO·5HO 有助于差异基因组 DNA 分离，系统发育分析表明，肯尼亚一株与 CCAP 1446/4 菌株和克隆的不同。附生细菌和的有机提取物未产生环二肽。对来自的 24 株厚壁菌的进一步表征鉴定出提取物为最活跃。我们的结果表明，在 ATCC 14579 中发现的微球菌 P1()的合成遗传基础取决于物种/菌株，这加强了在全球范围内对微生物物种及其附生物进行分子鉴定的必要性。这些发现表明，与相关的细菌是一种被忽视的抗菌化合物来源。
