Laboratory of Marine Drugs, School of Earth and Environmental Sciences, Seoul National University, NS-80, Seoul 08826, Korea.
Research Institute of Oceanography, Seoul National University, NS-80, Seoul 08826, Korea.
Molecules. 2020 Sep 24;25(19):4383. doi: 10.3390/molecules25194383.
A new thiopeptide (micrococcin P3, ) and a known one (micrococcin P1, ) were isolated from the culture broth of a marine-derived strain of . The structures of both compounds were elucidated using spectroscopic methods, including extensive 1D and 2D NMR analysis, high resolution mass spectrometry (HRMS), and tandem mass spectrometry. Both compounds exhibited potent antibacterial activities against Gram-positive strains with minimum inhibitory concentration (MIC) values of 0.05-0.8 μg/mL and did not show cytotoxicity in the MTT assay up to a concentration of 10 μM. This study adds a new promising member, micrococcin P3, to the family of thiopeptide antibiotics, which shows potential for the development of new antibiotics targeting Gram-positive bacteria.
从一株海洋来源的 中分离得到两种新的噻肽（micrococcin P3， ）和一种已知的噻肽（micrococcin P1， ）。通过包括广泛的 1D 和 2D NMR 分析、高分辨率质谱（HRMS）和串联质谱在内的光谱方法阐明了这两种化合物的结构。这两种化合物均表现出对革兰氏阳性菌株的强大抗菌活性，最低抑菌浓度（MIC）值为 0.05-0.8 μg/mL，在 MTT 测定中在 10 μM 浓度下没有显示细胞毒性。本研究为噻肽抗生素家族添加了一个新的有前途的成员 micrococcin P3，它显示出针对革兰氏阳性菌的新型抗生素的开发潜力。
