Prasse Tobias, Stratos Ioannis, Niehoff Anja, Christ Hildegard, Heck Vincent, Meyer Carolin, Mittlmeier Thomas
Department of Orthopedics and Trauma Surgery, Faculty of Medicine and University Hospital of Cologne, University of Cologne, Kerpener Str. 62, 50937 Cologne, Germany.
Department of Orthopedics, Würzburg University Medical Center, König-Ludwig-Haus, Brettreichstraße 11, 97074 Würzburg, Germany.
Toxics. 2022 Feb 14;10(2):86. doi: 10.3390/toxics10020086.
Bisphenol A (BPA), which is contained in numerous plastic products, is known to act as an endocrine-disruptive, toxic, and carcinogenic chemical. This experimental series sought to determine the influence of BPA exposure on the femoral bone architecture and biomechanical properties of male and female Wistar rats. BPA was applied subcutaneously by using osmotic pumps. After 12 weeks, the bones were analyzed by micro-computed tomography (micro-CT) and a three-point bending test. Comparing the female low- and high-dose groups, a significantly greater marrow area ( = 0.047) was identified in the group exposed to a higher BPA concentration. In addition, the trabecular number tended to be higher in the female high-dose group when compared to the low-dose group ( > 0.05). The area moment of inertia also tended to be higher in the male high-dose group when compared to the male low-dose group ( > 0.05). Considering our results, BPA-related effects on the bone morphology in female Wistar rats are osteoanabolic after high-dose exposure, while, in male rats, a tendency toward negative effects on the bone morphology in terms of a reduced cross-sectional cortical area and total area could be demonstrated.
双酚A（BPA）存在于众多塑料制品中，已知其具有内分泌干扰性、毒性和致癌性。本系列实验旨在确定暴露于双酚A对雄性和雌性Wistar大鼠股骨骨骼结构和生物力学特性的影响。通过渗透泵皮下注射双酚A。12周后，采用微型计算机断层扫描（micro-CT）和三点弯曲试验对骨骼进行分析。比较雌性低剂量组和高剂量组，在双酚A浓度较高的暴露组中，骨髓面积显著更大（P = 0.047）。此外，与低剂量组相比，雌性高剂量组的骨小梁数量有升高趋势（P > 0.05）。与雄性低剂量组相比，雄性高剂量组的惯性矩也有升高趋势（P > 0.05）。综合我们的研究结果，高剂量暴露后，双酚A对雌性Wistar大鼠骨骼形态的影响为骨合成代谢，而在雄性大鼠中，可证明在骨形态方面存在负面影响趋势，即横截面皮质面积和总面积减小。
