在猫咪收容所中通过三种快速抗原免疫层析试验和实时聚合酶链反应直接检测猫冠状病毒

Direct Detection of Feline Coronavirus by Three Rapid Antigen Immunochromatographic Tests and by Real-Time PCR in Cat Shelters.

作者信息

Vojtkovská Veronika, Lukešová Gabriela, Voslářová Eva, Konvalinová Jarmila, Večerek Vladimír, Lobová Dana

机构信息

Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Palackého tř. 1946/1, 612 42 Brno, Czech Republic.

Department of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary Sciences Brno, Palackého tř. 1946/1, 612 42 Brno, Czech Republic.

出版信息

Vet Sci. 2022 Jan 18;9(2):35. doi: 10.3390/vetsci9020035.

DOI:10.3390/vetsci9020035

PMID:35202289

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8875548/

Abstract

The aim of this study was the direct detection of feline coronavirus by real-time PCR and by three different rapid immunochromatographic (RIM) tests detecting antigens in faecal samples of shelter cats. Based on sensitivity and specificity calculated for each of the RIM tests, the utility of RIM tests was compared. Seventy faecal samples originating from shelter cats housed in quarantine were examined. Out of 70 samples analyzed by real-time PCR, 44 (62.9%) were positive. Significantly more cats ( < 0.05) tested positive than negative. Neither age nor sex of the cats played a significant role ( > 0.05) in the shedding status of the virus. The sensitivity of the RIM tests was found to be at low (<35%; RIM tests A and C) to satisfactory level (>50%, RIM test B). The number of virus particles determined by real-time RT-PCR analysis did not significantly correlate with the results detected by any of the RIM tests ( > 0.05). The results of this study indicate that the use of rapid antigen RIM tests in routine screening of FCoV shedding status in shelter cats is limited due to the occurrence of a high number of false negative results.

摘要

本研究的目的是通过实时聚合酶链反应（PCR）以及三种不同的快速免疫层析（RIM）试验直接检测猫冠状病毒，这三种试验用于检测收容所猫粪便样本中的抗原。根据为每种RIM试验计算的敏感性和特异性，对RIM试验的实用性进行了比较。对70份来自处于隔离期的收容所猫的粪便样本进行了检测。在通过实时PCR分析的70份样本中，44份（62.9%）呈阳性。检测呈阳性的猫显著多于呈阴性的猫（<0.05）。猫的年龄和性别在病毒排出状态方面均未发挥显著作用（>0.05）。发现RIM试验的敏感性处于低水平（<35%；RIM试验A和C）至令人满意的水平（>50%，RIM试验B）。通过实时逆转录PCR分析确定的病毒颗粒数量与任何一种RIM试验检测的结果均无显著相关性（>0.05）。本研究结果表明，由于出现大量假阴性结果，在收容所猫FCoV排出状态的常规筛查中使用快速抗原RIM试验受到限制。