Guo Shuyu, Gao Ge, Zhang Cuizhen, Peng Gang
State Key Laboratory of Medical Neurobiology, Ministry of Education Frontiers Center for Brain Science, and Institution of Brain Science, Fudan University, Shanghai 200032, China.
Vet Sci. 2022 Feb 19;9(2):92. doi: 10.3390/vetsci9020092.
Zebrafish are widely used to investigate candidate genes for human diseases. While the emergence of CRISPR-Cas9 technology has revolutionized gene editing, the use of individual guide RNAs limits the efficiency and application of this technology in functional genetics research. Multiplexed genome editing significantly enhances the efficiency and scope of gene editing. Herein, we describe an efficient multiplexed genome editing strategy to generate zebrafish mutants. Following behavioural tests and histological examination, we identified one new candidate gene () for hearing loss. This study provides a robust genetic platform to quickly obtain zebrafish mutants and to identify candidate genes by phenotypic readouts.
斑马鱼被广泛用于研究人类疾病的候选基因。虽然CRISPR-Cas9技术的出现彻底改变了基因编辑，但单个导向RNA的使用限制了该技术在功能遗传学研究中的效率和应用。多重基因组编辑显著提高了基因编辑的效率和范围。在此，我们描述了一种高效的多重基因组编辑策略来生成斑马鱼突变体。经过行为测试和组织学检查，我们鉴定出一个新的听力损失候选基因（）。本研究提供了一个强大的遗传平台，可快速获得斑马鱼突变体并通过表型读数鉴定候选基因。
