Chapuis Ronan J J, Becker Anne A M J, Dowling Patricia M, Weese J Scott
Clinical Sciences, Biomedical Sciences, Ross University School of Veterinary Medicine, Basseterre, Saint Kitts and Nevis.
Large Animal Clinical Sciences, Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Equine Vet J. 2023 Jan;55(1):129-141. doi: 10.1111/evj.13570. Epub 2022 Mar 14.
Antimicrobial-associated diarrhoea is a common adverse effect of antimicrobial treatment in horses and has been reported following the administration of oral doxycycline. The administration of antimicrobials has also been associated with changes in the equine intestinal microbiota diversity yet has not been explored under doxycycline treatment.
To describe the dynamics of the faecal microbial diversity following a 5-day oral administration of doxycycline in healthy horses with Streptococcus zooepidemicus infected tissue chambers.
Experimental prospective cohort study in a single horse group.
Seven healthy adult horses with S. zooepidemicus infected tissue chambers received oral doxycycline at 10 mg/kg q 12 h for 5-days following the tissue chamber inoculation. Faeces were collected prior to the tissue chamber inoculation and until 28-days post inoculation. Faecal microbiota was characterised by high throughput sequencing of the V4 region of the 16S rRNA gene on the Illumina MiSeq sequencing platform. Bioinformatic analysis was performed with Mothur and statistical analysis were conducted on R Studio.
A significant decrease in alpha diversity, characterised by a decrease of richness and diversity, and a decrease in beta diversity, characterised by changes in relative abundance, occurred after initiation of and during the administration of doxycycline. A decrease in Verrucomicrobia and increase in Firmicutes:Bacteroidetes ratio occurred following the initiation of treatment, with a return to initial Firmicutes:Bacteroidetes ratio during the treatment. It took 23 days after discontinuing the treatment for the faecal microbiota to return close to the initial state.
Lack of control population within the study.
Transitory intestinal dysbiosis occurs under oral administration of doxycycline in horses.
抗菌药物相关性腹泻是马抗菌治疗常见的不良反应，口服强力霉素后已有相关报道。抗菌药物的使用也与马肠道微生物群多样性的变化有关，但在强力霉素治疗下尚未进行过研究。
描述在感染兽疫链球菌组织腔的健康马匹中，口服强力霉素5天后粪便微生物多样性的动态变化。
单组马匹的实验性前瞻性队列研究。
7匹感染兽疫链球菌组织腔的健康成年马，在组织腔接种后，按10mg/kg的剂量每12小时口服强力霉素，持续5天。在组织腔接种前和接种后直至28天收集粪便。通过Illumina MiSeq测序平台对16S rRNA基因V4区域进行高通量测序来表征粪便微生物群。使用Mothur进行生物信息分析，并在R Studio上进行统计分析。
在强力霉素开始给药时及给药期间，以丰富度和多样性降低为特征的α多样性显著降低，以相对丰度变化为特征的β多样性也降低。治疗开始后疣微菌门减少，厚壁菌门与拟杆菌门的比例增加，治疗期间厚壁菌门与拟杆菌门的比例恢复到初始水平。停药后23天粪便微生物群才恢复到接近初始状态。
研究中缺乏对照群体。
马匹口服强力霉素会出现短暂的肠道菌群失调。
