粪便微生物移植对腹泻驹临床结局和粪便微生物的影响。

Effects of fecal microbiota transplantation on clinical outcomes and fecal microbiota of foals with diarrhea.

机构信息

Charles Sturt University School of Agricultural, Environmental and Veterinary Sciences, Wagga Wagga, New South Wales, Australia.

Scone Equine Hospital, Scone, New South Wales, Australia.

出版信息

J Vet Intern Med. 2024 Sep-Oct;38(5):2718-2728. doi: 10.1111/jvim.17185. Epub 2024 Sep 12.

DOI:10.1111/jvim.17185

PMID:39266472

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11423448/

Abstract

BACKGROUND

Diarrhea in foals can be associated with disruption of the intestinal microbiota (dysbiosis). Effective management of intestinal dysbiosis in foals has not been demonstrated.

HYPOTHESIS/OBJECTIVES: Fecal microbiota transplantation (FMT) in foals with diarrhea influences the intestinal microbiota and improves clinical and clinicopathological outcomes.

ANIMALS

Twenty-five foals <6 months of age with diarrhea and systemic inflammatory response syndrome at 3 veterinary hospitals.

METHODS

A prospective randomized placebo-controlled cohort study. Foals in the FMT group (n = 19) or control group (n = 9) received FMT or electrolyte solution once daily for 3 days. Fecal samples were obtained on Day 0 (D0), D1, D2, D3, and D7. Within group and between group data analyses were performed for clinical, clinicopathological, and microbiota variables.

RESULTS

Treatment had no effect on survival (FMT 79%; control 100%, P = .3) or resolution of diarrhea (FMT 68%; control 55%, P = .4). On D3, the white blood cell count of the FMT group was lower than the control group (D3 FMT group median 6.4 g/L [5-8.3 g/L]; D3 control group median 14.3 g/L [6.7-18.9 g/L] P = .04). Heart rate reduced over time in the FMT group (D0 median 80 bpm [60-150 bpm]; D2 median 70 bpm [52-110 bpm] [P = .005]; and D3 median 64, [54-102 bpm] [P < .001]). Phylum Verrucomicrobiota, genus Akkermansia, and family Prevotellaceae were enriched in the FMT group on D1 (linear discriminate analysis > 4).

CONCLUSIONS AND CLINICAL IMPORTANCE

In foals with diarrhea, FMT appears safe and can be associated with some clinical and microbiota changes suggestive of beneficial effect.

摘要

背景

幼驹腹泻可能与肠道微生物群失调（肠道生态失调）有关。尚未证实有效治疗幼驹肠道生态失调。

假设/目的：粪便微生物群移植（FMT）治疗腹泻幼驹可影响肠道微生物群，并改善临床和临床病理结局。

动物

3 家兽医医院的 25 头＜6 月龄、腹泻伴全身炎症反应综合征的幼驹。

方法

前瞻性随机安慰剂对照队列研究。FMT 组（n = 19）或对照组（n = 9）的幼驹每天接受 FMT 或电解质溶液治疗 3 天，1 次/d。于第 0 天（D0）、D1、D2、D3 和 D7 采集粪便样本。对临床、临床病理和微生物组变量进行组内和组间数据分析。

结果

治疗对存活率（FMT 组 79%；对照组 100%，P = .3）或腹泻缓解（FMT 组 68%；对照组 55%，P = .4）均无影响。在 D3，FMT 组的白细胞计数低于对照组（D3 FMT 组中位数 6.4 g/L[5-8.3 g/L]；D3 对照组中位数 14.3 g/L[6.7-18.9 g/L]，P = .04）。FMT 组的心率随时间逐渐降低（D0 中位数 80 bpm[60-150 bpm]；D2 中位数 70 bpm[52-110 bpm]，P = .005；D3 中位数 64，bpm[54-102 bpm]，P < .001）。FMT 组在 D1 时厚壁菌门、阿克曼氏菌属和普雷沃氏菌科富集（线性判别分析 > 4）。