Morgan T M, Sutanto I, Partono F, Maizels R M
Parasitology. 1986 Dec;93 ( Pt 3):559-69. doi: 10.1017/s0031182000081269.
Adult Wuchereria bancrofti were recovered from infected Presbytis cristatus monkeys and radio-isotope labelled extrinsically with 125I and in vitro with [35S]methionine. 125I labelling of the surface of adult W. bancrofti permitted a comparison between the major surface antigens of this species and those from the related lymphatic filariae, Brugia malayi and B. pahangi. All species bear a prominent Mr 29,000 surface antigen but among the differences observed were the strongly labelled molecules with Mr 58,000 and 67,000 in W. bancrofti which are extremely faint in the Brugia species. The [35S]methionine label was effectively incorporated into somatic parasite proteins in vitro although it was not possible to identify any secreted proteins in this way. The antigenicity of these products was investigated using a variety of sera from homologous and heterologous infections and the immunoprecipitation patterns highlighted particular differences between somatic proteins of male and female worms. One secreted antigen was detected, however, by virtue of its phosphorylcholine epitopes, in the culture medium of mixed adult worms; medium from male W. bancrofti adults was negative although homogenates of either sex of adult W. bancrofti were strongly positive in the same system.
从感染的冠叶猴体内获取成年班氏吴策线虫，用125I进行体外放射性同位素标记，并用[35S]甲硫氨酸进行体外标记。对成年班氏吴策线虫表面进行125I标记，以便比较该物种与相关淋巴丝虫马来布鲁线虫和彭亨布鲁线虫的主要表面抗原。所有物种都有一个突出的29,000道尔顿表面抗原，但观察到的差异包括班氏吴策线虫中58,000道尔顿和67,000道尔顿的强标记分子，而在布鲁线虫属物种中则极其微弱。[35S]甲硫氨酸标记物在体外有效地掺入到虫体蛋白质中，尽管无法用这种方法鉴定任何分泌蛋白。使用来自同源和异源感染的多种血清研究了这些产物的抗原性，免疫沉淀模式突出了雄虫和雌虫虫体蛋白质之间的特定差异。然而，通过其磷酸胆碱表位，在混合成年虫的培养基中检测到一种分泌抗原；成年雄班氏吴策线虫的培养基呈阴性，尽管成年班氏吴策线虫雌雄两性的匀浆在同一系统中呈强阳性。
