Goldshmit Yona, Shalom Moshe, Ruban Angela
Steyer School of Health Professions, Sackler Faculty of Medicine, Tel-Aviv University, Tel Aviv 6997801, Israel.
Australian Regenerative Medicine Institute, Monash Biotechnology, 15 Innovation Walk, Melbourne 3800, Australia.
Biomedicines. 2022 Jan 29;10(2):325. doi: 10.3390/biomedicines10020325.
Spinal cord injury (SCI) pathology includes both primary and secondary events. The primary injury includes the original traumatic event, and the secondary injury, beginning immediately after the initial injury, involves progressive neuroinflammation, neuronal excitotoxicity, gliosis, and degeneration. Currently, there is no effective neuroprotective treatment for SCI. However, an accumulating body of data suggests that PELF-EMF has beneficial therapeutic effects on neurotrauma. The purpose of this study was to test the efficacy of the PELF-EMF SEQEX device using a compression SCI mouse model.
C57BL/6 mice were exposed to PELF-EMF for 4 h on a daily basis for two months, beginning 2 h after a mild-moderate compression SCI.
The PELF-EMF treatment significantly diminished inflammatory cell infiltration and astrocyte activation by reducing Iba1, F4/80, CD68+ cells, and GAFP at the lesion borders, and increased pro-survival signaling, such as BDNF, on the neuronal cells. Moreover, the treatment exhibited a neuroprotective effect by reducing the demyelination of the axons of the white matter at the lesion's center.
Treatment with SEQEX demonstrated significant anti-inflammatory and neuroprotective effects. Considering our results, this safe and effective rehabilitative device, already available on the market, may provide a major therapeutic asset in the treatment of SCI.
脊髓损伤（SCI）的病理过程包括原发性和继发性事件。原发性损伤包括最初的创伤事件，而继发性损伤在初始损伤后立即开始，涉及进行性神经炎症、神经元兴奋性毒性、胶质增生和变性。目前，尚无针对脊髓损伤的有效神经保护治疗方法。然而，越来越多的数据表明，脉冲电磁场（PELF-EMF）对神经创伤具有有益的治疗作用。本研究的目的是使用压迫性脊髓损伤小鼠模型测试PELF-EMF SEQEX设备的疗效。
C57BL/6小鼠在轻度至中度压迫性脊髓损伤后2小时开始，每天接受PELF-EMF照射4小时，持续两个月。
PELF-EMF治疗通过减少损伤边界处的Iba1、F4/80、CD68+细胞和胶质纤维酸性蛋白（GFAP），显著减少了炎症细胞浸润和星形胶质细胞活化，并增加了神经元细胞上的促存活信号，如脑源性神经营养因子（BDNF）。此外，该治疗通过减少损伤中心白质轴突的脱髓鞘表现出神经保护作用。
SEQEX治疗显示出显著的抗炎和神经保护作用。鉴于我们的研究结果，这种市场上已有的安全有效的康复设备可能为脊髓损伤的治疗提供重要的治疗手段。
