Chen Lei, Wang Fei, Qu Su, He Xiaona, Zhu Yongxin, Zhou Yi, Yang Kunlong, Li Yong-Xin, Liu Man, Peng Xue, Tian Jun
School of Life Science, Jiangsu Normal University, Xuzhou 221116, China.
Antioxidants (Basel). 2022 Jan 18;11(2):178. doi: 10.3390/antiox11020178.
Vulvovaginal candidiasis (VVC) is one of the most frequent diseases induced by () during pregnancy, which results in enormous pain to women and their partners in daily life. Perillaldehyde (PAE), a natural monoterpenoid, has significant anti-microbial, anti-inflammatory and anti-oxidation effects. Reactive oxygen species (ROS) are key factors for the host to resist the invasion of fungi. However, excess ROS can cause additional damage independent of the pathogen itself, and the mechanism of ROS in VVC has not been investigated. In this murine study, we revealed that infection increased the expression of NADPH oxidase 2 (NOX2) and the content of malonaldehyde (MDA). inhibited the activity of antioxidant enzymes in the vagina, including superoxide dismutase (SOD), Catalase (CAT), glutathione peroxidase (GSH-PX) and heme oxygenase (HO-1), which were returned to normal levels after treatment with PAE. Furthermore, PAE inhibited the activities of Keap1 and promoted Nrf2 transfer from cytoplasm to nucleus, which were mediated by excessive accumulation of ROS in the VVC mice. In this study, we also indicated that PAE inhibited the apoptosis of vagina cells via Caspase 9- Caspase 7-PARP pathway and prevented the release of IL-1ꞵ in VVC mice. In summary, this study revealed that the treatment of VVC in mice with PAE might be mediated by inhibition of ROS, and established the therapeutic potential of PAE as an antifungal agent for the treatment of VVC.
外阴阴道念珠菌病（VVC）是孕期最常见的由（）引起的疾病之一，给女性及其伴侣的日常生活带来巨大痛苦。紫苏醛（PAE）是一种天然单萜类化合物，具有显著的抗菌、抗炎和抗氧化作用。活性氧（ROS）是宿主抵抗真菌入侵的关键因素。然而，过量的ROS可独立于病原体本身造成额外损伤，且ROS在VVC中的作用机制尚未得到研究。在本小鼠研究中，我们发现（）感染增加了烟酰胺腺嘌呤二核苷酸磷酸氧化酶2（NOX2）的表达和丙二醛（MDA）的含量。（）抑制了阴道中抗氧化酶的活性，包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-PX）和血红素加氧酶（HO-1），用PAE治疗后这些酶的活性恢复到正常水平。此外，PAE抑制了Keap1的活性并促进了Nrf2从细胞质转移到细胞核，这是由VVC小鼠中ROS的过度积累介导的。在本研究中，我们还表明PAE通过半胱天冬酶9-半胱天冬酶7-聚（ADP-核糖）聚合酶途径抑制阴道细胞凋亡，并阻止VVC小鼠中白细胞介素-1β的释放。总之，本研究表明用PAE治疗小鼠VVC可能是通过抑制ROS介导的，并确立了PAE作为治疗VVC的抗真菌剂的治疗潜力。
