Pop Anca, Bogdan Catalina, Fizesan Ionel, Iurian Sonia, Carpa Rahela, Bacali Cecilia, Vlase Laurian, Benedec Daniela, Moldovan Mirela L
Department of Toxicology, Faculty of Pharmacy, "Iuliu Hațieganu" University of Medicine and Pharmacy, 6 L. Pasteur Street, 400349 Cluj-Napoca, Romania.
Department of Dermopharmacy and Cosmetics, Faculty of Pharmacy, "Iuliu Hațieganu" University of Medicine and Pharmacy, 12 I. Creanga Street, 400010 Cluj-Napoca, Romania.
Antioxidants (Basel). 2022 Jan 24;11(2):218. doi: 10.3390/antiox11020218.
In this study, the biological activities of four extracts from by-products: two pomace extracts, white (WPE) and red (RPE), a canes extract (CE), and their combination (CoE), were evaluated, to be included in freeze-drying mouthwashes formulations. The cytocompatibility and anticancerous potential of the four extracts were tested on three cancerous cell lines, as well as the cytoprotective activity against nicotine-induced cytotoxicity and the antioxidant potential determined on a human gingival fibroblasts (HGF) cell line. Additionally, the anti-inflammatory activity and the antimicrobial activity against several microorganisms from the oral microbiome were tested. Freeze-dried mouthwashes with CoE were prepared and characterized, both as lyophilizates and after reconstitution. The four tested extracts showed the highest cytotoxicity on MDA-kb2 cell line. The antioxidant potential was demonstrated for WPE, RPE, CE, and CoE, both in non-stimulated and HO stimulated conditions. The four extracts reduced the levels of proinflammatory cytokines (IL-6, IL-8, and IL-1β) in a dose-dependent manner, confirming their anti-inflammatory activity. The antimicrobial activity of tested extracts was shown against pathogenic bacteria from the oral microbiome. Mouthwashes of CoE with poloxamer-407, xylitol, and different ratios of mannitol were prepared by freeze-drying leading to porous formulations with interesting mechanical properties and reconstitution times.
在本研究中，评估了来自副产品的四种提取物的生物活性：两种果渣提取物，白色（WPE）和红色（RPE），一种茎提取物（CE）及其组合（CoE），以纳入冻干漱口水配方中。在三种癌细胞系上测试了这四种提取物的细胞相容性和抗癌潜力，以及对尼古丁诱导的细胞毒性的细胞保护活性和在人牙龈成纤维细胞（HGF）系上测定的抗氧化潜力。此外，还测试了抗炎活性和对口腔微生物群中几种微生物的抗菌活性。制备并表征了含CoE的冻干漱口水，包括冻干物和复溶后的情况。四种测试提取物对MDA-kb2细胞系显示出最高的细胞毒性。在非刺激和HO刺激条件下，WPE、RPE、CE和CoE均表现出抗氧化潜力。四种提取物以剂量依赖性方式降低促炎细胞因子（IL-6、IL-8和IL-1β）的水平，证实了它们的抗炎活性。测试提取物对口腔微生物群中的病原菌具有抗菌活性。通过冻干制备了含CoE与泊洛沙姆-407、木糖醇和不同比例甘露醇的漱口水，得到了具有有趣机械性能和复溶时间的多孔制剂。
