Epure Alexandra, Pârvu Alina E, Vlase Laurian, Benedec Daniela, Hanganu Daniela, Oniga Ovidiu, Vlase Ana-Maria, Ielciu Irina, Toiu Anca, Oniga Ilioara
Department of Pharmacognosy, Faculty of Pharmacy, "Iuliu Hațieganu" University of Medicine and Pharmacy, 8 V. Babeș Street, 400012 Cluj-Napoca, Romania.
Department of Physiopathology, Faculty of Medicine, "Iuliu Hațieganu" University of Medicine and Pharmacy, 8 V. Babeș Street, 400012 Cluj-Napoca, Romania.
Pharmaceuticals (Basel). 2023 Feb 26;16(3):358. doi: 10.3390/ph16030358.
The present research investigated the in vivo anti-inflammatory and cardioprotective activities, as well as the antioxidant potential of tincture (TOT), in relation to the polyphenolic composition. Chromatographic and spectrophotometric techniques were used to determine the polyphenolic profile of TOT and the antioxidant activity was preliminarily assessed in vitro by DPPH• and FRAP spectrophotometric methods. The in vivo anti-inflammatory and cardioprotective activities were studied in rat turpentine-induced inflammation and in rat isoprenaline-induced myocardial infarction (MI) models. The main polyphenolic compound identified in TOT was cichoric acid. The oxidative stress determinations showed the capacity of the dandelion tincture not only to decrease the total oxidative stress (TOS), the oxidative stress index (OSI), and the total antioxidant capacity (TAC), but also the malondialdehide (MDA), thiols (SH), and nitrites/nitrates (NOx) levels both in inflammation and MI models. In addition, aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatin kinase-MB (CK-MB), and nuclear factor kappa B (NF-κB) parameters were decreased by the administration of the tincture. The results show that could be considered a valuable source of natural compounds with important benefits in pathologies linked to oxidative stress.
本研究调查了蒲公英酊剂（TOT）与多酚成分相关的体内抗炎和心脏保护活性以及抗氧化潜力。采用色谱和分光光度技术测定TOT的多酚谱，并通过DPPH•和FRAP分光光度法初步体外评估其抗氧化活性。在大鼠松节油诱导的炎症和大鼠异丙肾上腺素诱导的心肌梗死（MI）模型中研究了其体内抗炎和心脏保护活性。TOT中鉴定出的主要多酚化合物是菊苣酸。氧化应激测定表明，蒲公英酊剂不仅能够降低炎症和MI模型中的总氧化应激（TOS）、氧化应激指数（OSI）和总抗氧化能力（TAC），还能降低丙二醛（MDA）、硫醇（SH）和亚硝酸盐/硝酸盐（NOx）水平。此外，给予该酊剂可降低天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、肌酸激酶-MB（CK-MB）和核因子κB（NF-κB）参数。结果表明，蒲公英酊剂可被视为天然化合物的宝贵来源，在与氧化应激相关的疾病中具有重要益处。
