Theil Gerit, Bialek Joanna, Weiß Christine, Lindner Felix, Fornara Paolo
Medical Faculty of Martin Luther University Halle-Wittenberg, University Clinic and Outpatient Clinic for Urology, 06120 Halle (Saale), Germany.
Diagnostics (Basel). 2022 Feb 15;12(2):497. doi: 10.3390/diagnostics12020497.
Selecting a well-suited method for isolating/characterizing circulating tumor cells (CTCs) is challenging. Evaluating sensitive and specific markers for prostate cancer (PCa)-specific CTC identification and analysis is crucial. We used the CellCollector EpCAM-functionalized system (CC-EpCAM) and evaluated and developed a PCa-functionalized version (CC-PCa); we then compared CTC isolation techniques that exploit the physical and biological properties of CTCs. We established two cohorts of metastatic PCa patients (mPCa; 15 in cohort 1 and 10 in cohort 2). CTC cultivation experiments were conducted with two capturing methods (Ficoll and ScreenCell). The most sensitive detection rates and highest CTC counts were reached with the CC-PCa and ScreenCell system. Patients with ≥5 CTCs isolated with CC-EpCAM had an overall survival (OS) of 0.93 years, and patients with ≥5 CTCs isolated with CC-PCa had an OS of 1.5 years in cohort 1. Nevertheless, we observed the highest sensitivity and specificity for 24-month survival by the Ficoll with CD45 depletion and ScreenCell system with May-Grunwald Giemsa (MGG) staining. The EpCAM molecule is an essential factor related to OS for CTC isolation based on biological properties in mPCa patients. The best-suited CTC capture system is not limited to one characteristic of cells but adapted to downstream analysis.
选择一种适合的方法来分离/鉴定循环肿瘤细胞（CTC）具有挑战性。评估用于前列腺癌（PCa）特异性CTC鉴定和分析的敏感且特异的标志物至关重要。我们使用了细胞收集器EpCAM功能化系统（CC-EpCAM），并评估和开发了一种PCa功能化版本（CC-PCa）；然后我们比较了利用CTC物理和生物学特性的CTC分离技术。我们建立了两组转移性PCa患者队列（mPCa；队列1中有15例，队列2中有10例）。使用两种捕获方法（Ficoll和ScreenCell）进行了CTC培养实验。CC-PCa和ScreenCell系统达到了最敏感的检测率和最高的CTC计数。在队列1中，用CC-EpCAM分离出≥5个CTC的患者总生存期（OS）为0.93年，用CC-PCa分离出≥5个CTC的患者OS为1.5年。然而，我们观察到，通过Ficoll去除CD45和使用May-Grunwald Giemsa（MGG）染色的ScreenCell系统，对24个月生存期具有最高的敏感性和特异性。在mPCa患者中，基于生物学特性，EpCAM分子是与CTC分离的OS相关的一个重要因素。最适合的CTC捕获系统不限于细胞的一个特征，而是要适应下游分析。
