Mendes Furtado Mariely, Lima Rocha Joana Érica, da Silva Mendes Ana Victória, Mello Neto Renato Sampaio, Brito Ana Karolinne da Silva, Sena de Almeida José Otávio Carvalho, Rodrigues Queiroz Emerson Iuri, de Sousa França José Vinícius, Cunha Sales Ana Lina de Carvalho, Gomes Vasconcelos Andreanne, Felix Cabral Wanessa, de Oliveira Lopes Luana, Souza do Carmo Iolanda, Souza Kückelhaus Selma Aparecida, de Souza de Almeida Leite José Roberto, Nunes Adriana Maria Viana, Rizzo Marcia Dos Santos, Citó Antônia Maria das Graças Lopes, Fortes Lustosa Ana Karina Marques, Lucarini Massimo, Durazzo Alessandra, Martins Maria do Carmo de Carvalho E, Arcanjo Daniel Dias Rufino
Department of Biophysics and Physiology, Federal University of Piauí, Teresina 64049-550, PI, Brazil.
University Hospital, Federal University of Piauí, Teresina 64049-550, PI, Brazil.
Biology (Basel). 2022 Jan 27;11(2):202. doi: 10.3390/biology11020202.
Atherosclerosis is a cardiovascular disease associated with abnormalities of vascular functions. The consumption of mono- and polyunsaturated fatty acids can be considered a strategy to reduce clinical events related to atherosclerosis. In the present study, we investigated the effects of supplementation with 310 mg of ω-3 PUFAs (2:1 eicosapentaenoic/docosahexaenoic acids) for 56 days on rats with hypercholesterolemia induced by a diet containing cholesterol (0.1%), cholic acid (0.5%), and egg yolk. Serum biochemical parameters were determined by the enzymatic colorimetric method. Assessment of vascular effects was performed by analysis of histological sections of the heart and aortic arch stained with hematoxylin and eosin and vascular reactivity of the aorta artery. We observed that treatment with ω-3 PUFAs did not promote alterations in lipid profile. On the other hand, we documented a favorable reduction in liver biomarkers, as well as contributions to the preservation of heart and aortic arch morphologies. Interestingly, the vascular reactivity of rat thoracic aortic preparations was improved after treatment with ω-3 PUFAs, with a decrease in hyperreactivity to phenylephrine and increased vasorelaxation promoted by acetylcholine. Our findings suggest that the supplementation of hypercholesterolemic rats with ω-3 PUFAs promoted improvement in liver and vascular endothelial function as well as preserving heart and aortic tissue, reinforcing the early health benefits of ω-3 PUFAs in the development of atherosclerotic plaque and further related events.
动脉粥样硬化是一种与血管功能异常相关的心血管疾病。食用单不饱和脂肪酸和多不饱和脂肪酸可被视为一种减少与动脉粥样硬化相关临床事件的策略。在本研究中，我们调查了给由含胆固醇（0.1%）、胆酸（0.5%）和蛋黄的饮食诱导的高胆固醇血症大鼠补充310毫克ω-3多不饱和脂肪酸（二十碳五烯酸/二十二碳六烯酸比例为2:1）56天的效果。血清生化参数通过酶比色法测定。通过对用苏木精和伊红染色的心脏和主动脉弓组织切片进行分析以及对主动脉的血管反应性进行评估来评价血管效应。我们观察到用ω-3多不饱和脂肪酸治疗并未促进脂质谱的改变。另一方面，我们记录到肝脏生物标志物有良好的降低，以及对心脏和主动脉弓形态的保存有作用。有趣的是，用ω-3多不饱和脂肪酸治疗后大鼠胸主动脉制剂的血管反应性得到改善，对去氧肾上腺素的高反应性降低，乙酰胆碱促进的血管舒张增加。我们的研究结果表明，给高胆固醇血症大鼠补充ω-3多不饱和脂肪酸可促进肝脏和血管内皮功能的改善以及保护心脏和主动脉组织，强化了ω-3多不饱和脂肪酸在动脉粥样硬化斑块形成及进一步相关事件发展过程中的早期健康益处。
