Vázquez-Mosquera Juan M, Fernández-Novo Aitor, Bonet-Bo Martin, Pérez-Villalobos Natividad, Pesántez-Pacheco Jose L, Pérez-Solana Maria Luz, de Mercado Eduardo, Gardón Juan Carlos, Villagrá Arantxa, Sebastián Francisco, Pérez-Garnelo Sonia Salomé, Martínez Daniel, Astiz Susana
Medicine and Surgery Department, Veterinary Faculty, Complutense University of Madrid, Puerta de Hierro Avenue s/n, 28040 Madrid, Spain.
Department of Veterinary Medicine, School of Biomedical and Health Sciences, Universidad Europea de Madrid, C/Tajo s/n, 28670 Villaviciosa de Odón, Spain.
Biology (Basel). 2022 Jan 30;11(2):225. doi: 10.3390/biology11020225.
Multiple ovulation and embryo transfer (MOET) systems have been intensively implemented in Japanese Black cattle in Japan and to create Japanese Black herds out of these areas. Environmental conditions influence MOET efficiency. Thus, we describe results of 137 in vivo, non-surgical embryo flushings performed between 2016-2020, in a full-blood Japanese Black herd kept in Spain and the possible effects of heat, year, bull, donor genetic value, and metabolic condition. Additionally, 687 embryo transfers were studied for conception rate (CR) and recipient related factors. A total of 71.3% of viable embryos (724/1015) were obtained (5.3 ± 4.34/flushing). Donor metabolites did not affect embryo production ( > 0.1), although metabolite differences were observed over the years, and by flushing order, probably related to the donor age. CR was not affected by embryo type (fresh vs. frozen), recipient breed, and whether suckling or not suckling ( > 0.1). CR decreased significantly with heat (44.3 vs. 49.2%; ( = 0.042)) and numerically increased with recipient parity and ET-number. Pregnant recipients showed significantly higher levels of cholesterol-related metabolites, glucose, and urea ( < 0.05). Therefore, adequate MOET efficiency can be achieved under these conditions, and heat stress should be strongly avoided during Japanese Black embryo transfers. Moreover, recipients' metabolites are important to achieve pregnancy, being probably related to better nutrient availability during pregnancy.
在日本，多排卵和胚胎移植（MOET）系统已在日本黑牛中广泛应用，并在这些地区以外培育日本黑牛群。环境条件会影响MOET效率。因此，我们描述了2016年至2020年间在西班牙饲养的纯种日本黑牛群中进行的137次体内非手术胚胎冲洗的结果，以及热、年份、公牛、供体遗传价值和代谢状况可能产生的影响。此外，还研究了687次胚胎移植的受孕率（CR）和受体相关因素。共获得了71.3%的活胚（724/1015）（每次冲洗5.3±4.34个）。供体代谢产物不影响胚胎生产（P>0.1），尽管多年来以及按冲洗顺序观察到代谢产物存在差异，这可能与供体年龄有关。CR不受胚胎类型（新鲜胚胎与冷冻胚胎）、受体品种以及是否哺乳的影响（P>0.1）。随着热应激的出现，CR显著降低（44.3%对49.2%；P=0.042），并且随着受体胎次和胚胎移植次数的增加而略有上升。怀孕受体的胆固醇相关代谢产物、葡萄糖和尿素水平显著更高（P<0.05）。因此，在这些条件下可以实现足够的MOET效率，并且在日本黑牛胚胎移植期间应强烈避免热应激。此外，受体的代谢产物对于实现妊娠很重要，这可能与妊娠期间更好的营养供应有关。
