Kyndt Elliot C, Kyndt John A
College of Science and Technology, Bellevue University, Bellevue, NE 68005, USA.
Insects. 2022 Feb 11;13(2):190. doi: 10.3390/insects13020190.
We sequenced the complete mitogenomes, 18S and 28S rRNA of two new isolates, collected in Eastern Nebraska and Northern Arkansas (US). Based on molecular sequence data comparison and morphological characteristics, the new isolates were identified as a subspecies of Fabricius 1775, for which we propose the subspecies names '' and ''. The new 18S and 28S rRNA sequences were found to be 99% and 98% identical to . There are no other 18S or 28S rRNA sequences in the Genbank database, however, phylogenetic analysis of the Cox1 genes showed Chaudoir, 1843, and Morawitz, 1863, as the closest relatives. This is the first report of a mitogenome for , and only the second mitogenome for that genus. The nucleotide sequence identity between the mitogenomes of the two isolates is 98.8%, while the earlier sequenced Forster 1771 mitogenome is more distantly related, with only 90% (to ssp. ) and 89% (to ssp. ) overall nucleotide sequence identity. These new mitogenomes, and their phylogenetic analysis, firmly establish the position of on the Carabidae family tree and further refine the genus. In addition to the molecular data provided for the species, this approach also allowed us to identify bacterial and viral signatures for , , , and a giant virus, associated with the species. We hereby present a simple and efficient protocol for identification and phylogenetic analysis of , that is applicable to other Coleoptera, based on total DNA extraction and Illumina short-read Next-Gen sequencing.
我们对从美国内布拉斯加州东部和阿肯色州北部采集的两个新分离株的完整线粒体基因组、18S和28S rRNA进行了测序。基于分子序列数据比较和形态特征，新分离株被鉴定为Fabricius 1775的一个亚种，我们为此提出亚种名称“”和“”。发现新的18S和28S rRNA序列与……分别有99%和98%的同一性。Genbank数据库中没有其他18S或28S rRNA序列，然而，对Cox1基因的系统发育分析表明，1843年的Chaudoir和1863年的Morawitz是其最亲近的亲属。这是关于……线粒体基因组的首次报道，也是该属的第二个线粒体基因组报道。两个分离株线粒体基因组之间的核苷酸序列同一性为98.8%，而较早测序的Forster 1771线粒体基因组的亲缘关系更远，与……亚种的总体核苷酸序列同一性仅为90%，与……亚种为89%。这些新的线粒体基因组及其系统发育分析，牢固地确立了……在步甲科家族树中的位置，并进一步完善了该属。除了为……物种提供的分子数据外，这种方法还使我们能够识别与……物种相关的……、……、……的细菌和病毒特征以及一种巨型病毒。我们在此提出一种简单有效的……鉴定和系统发育分析方案，该方案基于总DNA提取和Illumina短读长新一代测序，适用于其他鞘翅目昆虫。
