Kelbich Petr, Vachata Petr, Maly Vilem, Novotny Tomas, Spicka Jan, Matuchova Inka, Radovnicky Tomas, Stanek Ivan, Kubalik Jan, Karpjuk Ondrej, Smisko Frantisek, Hanuljakova Eva, Krejsek Jan
Department of Biomedicine and Laboratory Diagnostics, Faculty of Health Studies, Jan Evangelista Purkinje University and Masaryk Hospital, 401 13 Usti nad Labem, Czech Republic.
Department of Clinical Immunology and Allergology, Faculty of Medicine and University Hospital, Charles University in Prague, 500 03 Hradec Kralove, Czech Republic.
Life (Basel). 2022 Jan 21;12(2):160. doi: 10.3390/life12020160.
The simultaneous cytological and metabolic investigation of various extravascular body fluids (EBFs) provides clinically relevant information about the type and intensity of the immune response in particular organ systems. The oxidative burst of professional phagocytes with the concomitant production of reactive oxygen species consumes a large amount of oxygen and is the cause of switch to the development of anaerobic metabolism. We assessed the relationships between percentages of neutrophils, aerobic and anaerobic metabolism, and tissue damage via the determination of aspartate aminotransferase catalytic activities (AST) in cerebrospinal fluid (CSF), pleural effusions (PE), abdominal effusions (AE), and synovial fluids (SF). 83.0% aerobic and 1.3% strongly anaerobic cases with median of AST = 13.8 IU/L in CSF; 68.0% aerobic and 9.0% strongly anaerobic cases with median of AST = 20.4 IU/L in PE; 77.5% aerobic and 10.5% strongly anaerobic cases with median of AST = 18.0 IU/L in AE; 64.1% aerobic and 7.7% strongly anaerobic cases with median of AST = 13.8 IU/L in SF. 4.2% aerobic and 73.7% strongly anaerobic cases with median of AST = 19.2 IU/L in CSF; 7.4% aerobic and 77.3% strongly anaerobic cases with median of AST = 145.2 IU/L in PE; 11.8% aerobic and 73.7% strongly anaerobic cases with median of AST = 61.8 IU/L in AE; 25.5% aerobic and 38.2% strongly anaerobic cases with median of AST = 37.2 IU/L in SF. The significant presence of neutrophils, concomitant strong anaerobic metabolism, and elevated AST in various EBFs are reliable signs of damaging purulent inflammation.
对各种血管外体液（EBF）进行细胞学和代谢同步研究，可为特定器官系统免疫反应的类型和强度提供临床相关信息。专业吞噬细胞的氧化爆发以及伴随产生的活性氧会消耗大量氧气，这是转向厌氧代谢发展的原因。我们通过测定脑脊液（CSF）、胸腔积液（PE）、腹腔积液（AE）和滑液（SF）中天冬氨酸转氨酶催化活性（AST），评估了中性粒细胞百分比、有氧和无氧代谢与组织损伤之间的关系。脑脊液中，83.0%为有氧情况，1.3%为强厌氧情况，AST中位数为13.8 IU/L；胸腔积液中，68.0%为有氧情况，9.0%为强厌氧情况，AST中位数为20.4 IU/L；腹腔积液中，77.5%为有氧情况，10.5%为强厌氧情况，AST中位数为18.0 IU/L；滑液中，64.1%为有氧情况，7.7%为强厌氧情况，AST中位数为13.8 IU/L。脑脊液中，4.2%为有氧情况，73.7%为强厌氧情况，AST中位数为19.2 IU/L；胸腔积液中，7.4%为有氧情况，77.3%为强厌氧情况，AST中位数为145.2 IU/L；腹腔积液中，11.8%为有氧情况，73.7%为强厌氧情况，AST中位数为61.8 IU/L；滑液中，25.5%为有氧情况，38.2%为强厌氧情况，AST中位数为37.2 IU/L。各种EBF中中性粒细胞的显著存在、伴随的强厌氧代谢以及AST升高是化脓性炎症损伤的可靠迹象。
