Bucková B, Hurníková Z, Lauková A, Revajová V, Dvorožňáková E
Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 04001 Košice, Slovakia.
Institute of Animal Physiology - Centre of Biosciences, Slovak Academy of Sciences, Šoltésovej 4, 040 01 Košice, Slovakia.
Helminthologia. 2018 Jun 1;55(2):102-111. doi: 10.2478/helm-2018-0010. eCollection 2018 Jun.
A potential protective effect of probiotic strains against zoonotic infection was investigated in the framework of a new therapeutic strategy aimed at using probiotics to control parasitic zoonoses. The study was focused on the impact of six selected probiotic (bacteriocinogenic) strains on the intensity of . infection and female fecundity and . Bacterial strains of different origin ( EF55, 2019 = CCM7420, AL41 = CCM8558, ED26E/7, AD1 = CCM7421, 17L/1) were administered daily in a dose of 10 CFU/ml in 100 μl, and mice were infected with 400 . larvae on day 7 of treatment. Female adults of . were isolated on day 5 post infection (p.i.) and subsequently were used in fecundity test . . CCM8558, . CCM7420 and . ED26E/7 strains significantly reduced the number of adults in the intestine. The application of . CCM7421, . 17L/1, . CCM8558 and . ED26E/7 caused a significant decrease in the number of muscle larvae. The treatment with . CCM8558 and . ED26E/7 showed the highest inhibitory effect on female fecundity (94 %). The number of newborn larvae (NBL) was also significantly decreased after administration of . CCM7421 and . 17L/1 (80 %). A direct impact of probiotic strains on female reproductive capacity was examined in females isolated from untreated infected mice on day 5 p.i. A correlation was found between the inhibitory effect and the concentration of probiotic strains. The reduction effects of the strains manifested as follows: . CCM7421 (93 %), . CCM8558, . 17L/1, . EF55 (about 80 %), . CCM7420 and . ED26E/7 (about 60 %).
在一项旨在利用益生菌控制寄生性人畜共患病的新治疗策略框架内，研究了益生菌菌株对人畜共患病感染的潜在保护作用。该研究聚焦于六种选定的益生菌（产细菌素）菌株对感染强度、雌虫繁殖力以及[此处原文缺失相关内容]的影响。不同来源的细菌菌株（EF55、2019 = CCM7420、AL41 = CCM8558、ED26E/7、AD1 = CCM7421、17L/1）每天以10⁸CFU/ml的剂量溶于100μl溶液中给药，在治疗第7天给小鼠感染400条[此处原文缺失相关寄生虫名称]幼虫。感染后第5天（p.i.）分离出[此处原文缺失相关寄生虫名称]雌成虫，随后用于繁殖力测试。CCM8558、CCM7420和ED26E/7菌株显著减少了肠道内成虫数量。CCM7421、17L/1、CCM8558和ED26E/7的应用使肌幼虫数量显著减少。CCM8558和ED26E/7处理对雌虫繁殖力的抑制作用最高（94%）。给予CCM7421和17L/1后，新生幼虫数量（NBL）也显著减少（80%）。在感染后第5天从未经处理的感染小鼠中分离出的雌虫中，检测了益生菌菌株对雌虫生殖能力的直接影响。发现抑制作用与益生菌菌株浓度之间存在相关性。菌株的降低作用表现如下：CCM7421（93%）、CCM8558、17L/1、EF55（约80%）、CCM7420和ED26E/7（约60%）。
