Bel'skaya Lyudmila V, Sarf Elena A, Solomatin Denis V, Kosenok Victor K
Biochemistry Research Laboratory, Omsk State Pedagogical University, 14, Tukhachevsky str., 644043 Omsk, Russia.
Department of Mathematics and Mathematics Teaching Methods, Omsk State Pedagogical University, 14, Tukhachevsky str., 644043 Omsk, Russia.
Metabolites. 2022 Feb 10;12(2):166. doi: 10.3390/metabo12020166.
The aim of the work was to study the metabolic characteristics of saliva in breast cancer and the subsequent assessment of the potential information content of its individual biochemical indicators. The study included 487 patients of the Omsk Clinical Oncology Center with morphologically verified breast cancer and 298 volunteers without breast pathologies. Saliva samples were obtained from all patients before the start of treatment, and the values of 34 biochemical indicators were determined. It has been shown that concentration of total protein, urea, uric acid (UA), the total content of α-amino acids and lipid peroxidation products, and the activity of metabolic and antioxidant enzymes (in particular catalase-CAT) of saliva changed significantly in breast cancer. Biochemical indicators characterizing early breast cancer have been identified, which can be used for timely diagnosis in addition to existing methods. The coefficients UA/Urea and UA·CAT/Urea are proposed, for which the maximum deviation from the norm was observed in the early stages of the disease. It was shown that for ductal breast cancer, changes in the activity of metabolic enzymes of saliva were more pronounced, while, for lobular breast cancer, the indicators of enzymatic and non-enzymatic components of antioxidant protection changed. The results confirmed the potential importance of saliva in the diagnosis of breast cancer.
这项工作的目的是研究乳腺癌患者唾液的代谢特征，并随后评估其各个生化指标的潜在信息含量。该研究纳入了487名经形态学证实患有乳腺癌的鄂木斯克临床肿瘤中心患者以及298名无乳腺病变的志愿者。在所有患者治疗开始前采集唾液样本，并测定34种生化指标的值。结果表明，乳腺癌患者唾液中的总蛋白、尿素、尿酸（UA）、α-氨基酸和脂质过氧化产物的总含量以及代谢和抗氧化酶（特别是过氧化氢酶-CAT）的活性发生了显著变化。已确定了表征早期乳腺癌的生化指标，除现有方法外，这些指标可用于早期诊断。提出了UA/尿素和UA·CAT/尿素系数，在疾病早期观察到其与正常水平的最大偏差。结果表明，对于导管癌，唾液代谢酶活性的变化更为明显，而对于小叶癌，抗氧化保护的酶促和非酶促成分指标发生了变化。结果证实了唾液在乳腺癌诊断中的潜在重要性。
