Laboratory of Pharmacology, School of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, Greece.
Division of Pharmacognosy and Natural Products Chemistry, School of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, Greece.
Molecules. 2022 Feb 11;27(4):1221. doi: 10.3390/molecules27041221.
Hot flashes are considered the most bothersome complaint during menopause. Although hormone therapy is an effective option to relieve hot flashes, it has been associated with significant side effects. The aim of our study is to suggest a novel combination of different plant extracts with distinct mechanisms of action against hot flashes. We selected the rhizome of L. (Fabaceae), the rhizome of L. (Ranunculaceae), the aerial parts of L. (Hypericaceae) to produce extracts rich in bioactive phytochemicals and the seed oil of L. (Onagraceae). We investigated their estrogenic and antioxidant potential and their inhibitory effect against prostaglandin D2 receptor 1 (DP1) as a novel mechanistic pathway for vasodilation in hot flashes, alone or in combination. The phytochemical footprint of the extracts was analyzed using HPLC-PDA and UPLC-HRMS. We observed that the tested extracts possess different mechanisms of action. exerts a beneficial activation of the estrogen receptor, possesses the highest antioxidant capacity and the seed oil of inhibits the DP1 receptor. The triple combination in the optimal doses pertains to efficacy against all three mechanisms of action, serves as a multitarget plant-based therapy and could serve as a novel strategy for the alleviation of hot flashes in postmenopausal women.
热潮是围绝经期最困扰的症状之一。尽管激素治疗是缓解热潮的有效选择，但它与显著的副作用有关。我们的研究目的是提出一种新的植物提取物组合，这些提取物具有不同的作用机制，可以对抗热潮。我们选择了 L.（豆科）的根茎、 L.（毛茛科）的根茎、 L.（藤黄科）的地上部分来生产富含生物活性植物化学物质的提取物和 L.（柳叶菜科）的种子油。我们研究了它们的雌激素和抗氧化潜力，以及它们对前列腺素 D2 受体 1（DP1）的抑制作用，作为热潮中血管舒张的新机制途径，单独或联合使用。使用 HPLC-PDA 和 UPLC-HRMS 分析提取物的植物化学特征。我们观察到，测试的提取物具有不同的作用机制。 对雌激素受体具有有益的激活作用， 具有最高的抗氧化能力，而种子油 则抑制 DP1 受体。最佳剂量的三联组合针对所有三种作用机制具有疗效，是一种多靶点植物性治疗方法，可作为缓解绝经后妇女热潮的新策略。
