Opt Express. 2022 Feb 14;30(4):4856-4866. doi: 10.1364/OE.442043.
We describe a computational light-sheet microscope designed for hyperspectral acquisition at high spectral resolution. The fluorescence light emitted from the full field-of-view is focused along the entrance slit of an imaging spectrometer using a cylindrical lens. To acquire the spatial dimension orthogonal to the slit of the spectrometer, we propose to illuminate the specimen with a sequence of structured light patterns and to solve the image reconstruction problem. Beam shaping is obtained simply using a digital micromirror device in conjunction with a traditional selective plane illumination microscopy setup. We demonstrate the feasibility of this method and report the first results in vivo in hydra specimens labeled using two fluorophores.
我们描述了一种计算光片显微镜，专为高光谱分辨率的高光谱采集而设计。使用柱面透镜将来自全视场的荧光光聚焦在成像光谱仪的入口狭缝上。为了获取与光谱仪狭缝正交的空间维度，我们建议用一系列结构光图案照射标本，并解决图像重建问题。通过结合传统的选择平面照明显微镜设置使用数字微镜器件，可以简单地获得光束整形。我们证明了这种方法的可行性，并报告了使用两种荧光染料标记的水螅标本的体内的初步结果。
