Medical Faculty, Kunming University of Science and Technology, Kunming, China.
Endocrinology Branch, The First People's Hospital of Yunnan Province, Kunming, China.
Front Cell Infect Microbiol. 2022 Feb 8;11:804733. doi: 10.3389/fcimb.2021.804733. eCollection 2021.
The diabetic autonomic neuropathy is one of the most common complications in type 2 diabetes mellitus (T2DM), especially gastrointestinal autonomic neuropathy (GAN), which occurs in up to 75% of patients. The study aimed to investigate the gut microbiota composition, structure, and function in T2DM patients with GAN (T2DM_GAN) and set up a link between gut microbiota and clinical characteristics of patients.
DNA was extracted from fecal samples of three groups using the kit method: healthy volunteers (n = 19), the patients with T2DM (n = 76), and T2DM_GAN (n = 27). Sequencing of 16S ribosomal DNA was performed using the MiSeq platform.
According to the clinical data, higher age, lower triglyceride, and lower body mass index were the main features of patients with T2DM_GAN. The gut microbiota analysis showed that Bacteroidetes, Firmicutes, and Proteobacteria constituted the three dominant phyla in healthy individuals. In addition, the gut microbiota structure and function of T2DM_GAN patients were clearly different from that of T2DM patients. T2DM patients were characterized by Fusobacteria, Fusobacteriia, Fusobacteriales, Fusobacteriaceae, , , and . Those gut microbiota may be involved in carotenoid and flavonoid biosyntheses. Relatively, the Gammaproteobacteria, Enterobacteriales, Enterobacteriaceae, , , , and were characteristic in the T2DM_GAN patients. Those may be involved in bacterial invasion of epithelial cells and pathogenic infection.
GAN exacerbated gut microbiota dysbiosis in adult patients with T2DM. The findings indicated that phyla Fusobacteria and class Gammaproteobacteria were closely related to the occurrence of T2DM. Especially the latter may promote T2DM_GAN.
糖尿病自主神经病变是 2 型糖尿病（T2DM）最常见的并发症之一，尤其是胃肠道自主神经病变（GAN），在多达 75%的患者中发生。本研究旨在探讨 T2DM 伴 GAN（T2DM_GAN）患者的肠道微生物群落组成、结构和功能，并建立肠道微生物群与患者临床特征之间的联系。
采用试剂盒法从三组粪便样本中提取 DNA：健康志愿者（n=19）、T2DM 患者（n=76）和 T2DM_GAN 患者（n=27）。使用 MiSeq 平台对 16S 核糖体 DNA 进行测序。
根据临床资料，T2DM_GAN 患者的主要特征为年龄较高、甘油三酯较低、体重指数较低。肠道微生物群分析表明，Bacteroidetes、Firmicutes 和 Proteobacteria 构成健康个体的三个主要门。此外，T2DM_GAN 患者的肠道微生物群结构和功能明显不同于 T2DM 患者。T2DM 患者的特征为 Fusobacteria、Fusobacteriia、Fusobacteriales、Fusobacteriaceae、、和。这些肠道微生物群可能参与类胡萝卜素和类黄酮的生物合成。相对地，T2DM_GAN 患者的特征为 Gammaproteobacteria、Enterobacteriales、Enterobacteriaceae、、、、和。这些可能参与细菌侵袭上皮细胞和致病性感染。
GAN 加重了成年 T2DM 患者肠道微生物群的失调。研究结果表明，Fusobacteria 门和 Gammaproteobacteria 纲与 T2DM 的发生密切相关。特别是后者可能促进 T2DM_GAN 的发生。
