Genome Sciences Center, BC Cancer Agency, Vancouver, British Columbia, V5Z 1L3, Canada.
Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
Microb Genom. 2020 Feb;6(2). doi: 10.1099/mgen.0.000300. Epub 2019 Oct 29.
Here, we report comprehensive transcriptomic profiles from under conditions that mimic the first stages of bacterial infection in a highly differentiated adenocarcinoma epithelial cell line. Our transcriptomic adenocarcinoma approach allows us to measure the expression dynamics and regulation of bacterial virulence and response factors in real time, and is a novel strategy for clarifying the role of infection in colorectal cancer (CRC) progression. Our data show that: (i) infection alters metabolic and functional pathways in , allowing the bacterium to adapt to the host-imposed milieu; (ii) infection also stimulates the expression of genes required to help induce and promote a hypoxic and inflammatory microenvironment in the host; and (iii) invasion occurs by a haematogenous route of infection. Our study identifies novel gene targets from that are activated during invasion and which may aid in determining how this species invades and promotes disease within the human gastrointestinal tract. These invasion-specific genes may be useful as biomarkers for CRC progression in a host and could also assist in the development of new diagnostic tools and treatments (such as vaccines or small molecule drug targets), which will be able to combat infection and inflammation in the host while circumventing the potential problem of tolerization.
在这里，我们报告了综合转录组谱，这些谱是在高度分化的腺癌细胞系中模拟细菌感染的最初阶段的条件下获得的。我们的腺癌细胞转录组学方法使我们能够实时测量细菌毒力和反应因子的表达动态和调控，这是阐明感染在结直肠癌（CRC）进展中的作用的一种新策略。我们的数据表明：（i）感染改变了细菌的代谢和功能途径，使细菌能够适应宿主环境；（ii）感染还刺激了宿主诱导和促进缺氧和炎症微环境所需基因的表达；（iii）感染通过血液途径发生。我们的研究鉴定了在入侵过程中被激活的新型来自的基因靶标，这可能有助于确定该物种如何在人类胃肠道中入侵和促进疾病。这些入侵特异性基因可作为宿主中 CRC 进展的生物标志物，也可用于开发新的诊断工具和治疗方法（如疫苗或小分子药物靶点），这些方法能够在宿主中对抗感染和炎症，同时避免 耐受化的潜在问题。
