Bazanova E A, Nechaeva N P, Lavrent'eva N N, Bukhova V P
Zh Mikrobiol Epidemiol Immunobiol. 1986 Apr(4):61-5.
The present work deals with a modification of the cytotoxic test for the determination of the cytotoxic activity of lymphocytes in infectious diseases. This modification is based on the use of the suspension of mononuclear blood cells, simultaneously containing effector cells (sensitized lymphocytes) and target cells (autologous monocytes). The cytotoxic effect on monocytes is observed after the preliminary incubation of nonadhering cells (lymphocytes) with the antigen of microorganisms causing the infectious process. A statistically significant increase in the cytotoxic activity of lymphocytes was recorded in patients with primary erysipelas at the acute period of the disease. The cytotoxic effect has been found to persist at a high level for two weeks. By the end of the disease this effect drops to the level characteristic of clinically normal persons. An elevated level of the cytotoxic activity of lymphocytes in the presence of streptococcal antigens of one type has been detected in 72% of patients with primary erysipelas. This indicates that type-nonspecific streptococcal antigens take part in the formation of delayed hypersensitivity, which is also confirmed by the data obtained in animal experiments.
本研究涉及对细胞毒性试验的改进，用于测定传染病中淋巴细胞的细胞毒性活性。这种改进基于使用单核血细胞悬液，该悬液同时含有效应细胞（致敏淋巴细胞）和靶细胞（自体单核细胞）。在用引起感染过程的微生物抗原对非黏附细胞（淋巴细胞）进行预孵育后，观察到对单核细胞的细胞毒性作用。在原发性丹毒急性期患者中，淋巴细胞的细胞毒性活性有统计学意义的增加。已发现细胞毒性作用在高水平持续两周。到疾病末期，这种作用降至临床正常人的特征水平。在72%的原发性丹毒患者中检测到在一种链球菌抗原存在下淋巴细胞的细胞毒性活性升高。这表明非特异性链球菌抗原参与了迟发型超敏反应的形成，动物实验获得的数据也证实了这一点。
