Bazanova E A, Smirnova M N, Sovetova V P
Zh Mikrobiol Epidemiol Immunobiol. 1976(3):115-20.
A study was made (in vitro) of the cytotoxic action on the mouse L-cells of lymph otoxin obtained in cultivation with a thermostable fraction of streptococcus of the lymph node cells of guinea pigs with hypersensitivity of delayed type to the streptococcus antigens. The cytotoxic action was determined by the calculation of the number of the L-cells preserved in the monolayer after the action on it of a decanted fluid containing lymphotoxin. A marked cytotoxic action on the L-cells was produced only by decanted fluids obtained in cultivation of lymph node cells of guinea pigs sensitized by streptococcus culture with a specific antigen. In cultivation of lymphoid cells of the same animals with a nonspecific antigen (tuberculin) the decanted fluids produced no significant cytotoxic action on mous fibroblasts. The value of the cytotoxic effect depended on the dose of the thermostable fraction, with which the lymph node cells were incubated in the preparation of the decanted fluid, and also on the concentration of the lymph node cells in the initial suspension.
对豚鼠淋巴结细胞进行培养，获得对链球菌抗原具有迟发型超敏反应的热稳定部分链球菌，以此培养得到的淋巴毒素对小鼠L细胞的细胞毒性作用进行了体外研究。通过计算含有淋巴毒素的倾析液作用于单层L细胞后，单层中保存的L细胞数量来确定细胞毒性作用。仅由用特定抗原经链球菌培养致敏的豚鼠淋巴结细胞培养获得的倾析液对L细胞产生显著的细胞毒性作用。用非特异性抗原（结核菌素）培养同一动物的淋巴细胞时，倾析液对小鼠成纤维细胞无明显细胞毒性作用。细胞毒性作用的值取决于在制备倾析液时用于孵育淋巴结细胞的热稳定部分的剂量，也取决于初始悬浮液中淋巴结细胞的浓度。
