Koprowska I, Zipfel S, Ross A H, Herlyn M
Acta Cytol. 1986 May-Jun;30(3):207-13.
Six monoclonal antibodies, generated by immunization of mice with human cervical carcinoma cells maintained in tissue culture or with cells from fresh tumor tissue, reacted specifically with the malignant cells in 71% to 90% of the tumor tissue imprints and cervical smears containing neoplastic cells but not with normal cervical epithelial cells in smears from 21 to 23 healthy donors. Antibody CE 402 bound to epithelial cells associated with regeneration in 2 of the 23 normal smears tested. Considerable heterogeneity of antibody binding by malignant cells was observed. Antibody CE 400 was the most reactive, binding to more than 50% of the tumor cells in all reactive specimens. Five of these monoclonal antibodies detected protein antigens in the 80 K to 110 K molecular weight range. Our studies demonstrate the feasibility of producing monoclonal antibodies with selected specificity for cervical carcinoma. These antibodies may be of considerable diagnostic value.
通过用人宫颈癌组织培养细胞或新鲜肿瘤组织细胞免疫小鼠产生的六种单克隆抗体，能与71%至90%的肿瘤组织印片及含有肿瘤细胞的宫颈涂片中的恶性细胞发生特异性反应，但不与21至23名健康供者涂片的正常宫颈上皮细胞发生反应。在所检测的23份正常涂片中，抗体CE 402与2份与再生相关的上皮细胞结合。观察到恶性细胞抗体结合存在相当大的异质性。抗体CE 400反应性最强，在所有反应性标本中与超过50%的肿瘤细胞结合。其中五种单克隆抗体检测到分子量在80K至110K范围内的蛋白质抗原。我们的研究证明了产生对宫颈癌具有选定特异性的单克隆抗体的可行性。这些抗体可能具有相当大的诊断价值。
