University Research Institute for Biotechnology and Biomedicine (BIOTECMED), Universidad de Valencia, Valencia, Spain.
Translational Genomics Group, Incliva Health Research Institute, Valencia, Spain.
Methods Mol Biol. 2022;2434:207-215. doi: 10.1007/978-1-0716-2010-6_13.
Western blot assays are not adequate for high-throughput screening of protein expression because it is an expensive and time-consuming technique. Here we demonstrate that quantitative dot blots in plate format are a better option to determine the absolute contents of a given protein in less than 48 h. The method was optimized for the detection of the Muscleblind-like 1 protein in patient-derived myoblasts treated with a collection of more than 100 experimental oligonucleotides.
Western blot 检测不适用于蛋白质表达的高通量筛选，因为它是一种昂贵且耗时的技术。在这里，我们证明了板式定量点印迹是一种更好的选择，可以在不到 48 小时内确定给定蛋白质的绝对含量。该方法针对用超过 100 种实验性寡核苷酸处理的患者来源的成肌细胞中肌肉盲样蛋白 1 的检测进行了优化。
