Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming, China.
Hum Vaccin Immunother. 2021 Dec 2;17(12):5656-5665. doi: 10.1080/21645515.2021.2011548.
Live-attenuated rotavirus vaccine has shown low protection in underdeveloped or developing countries. However, the inactivated rotavirus vaccine may have the potential to overcome some of these challenges. In the present study, the immunogenicity and protective efficacy of a bivalent inactivated rotavirus vaccine by parenteral administration were elevated in a neonatal rhesus monkey model. A bivalent inactivated rotavirus vaccine containing G1P[8] (ZTR-68 strain) and G9P[8] (ZTR-18 strain) was administered to pregnant rhesus monkeys twice at an interval of 14 days. Neutralizing antibodies against RV strains ZTR-68, ZTR-18, SA11, WA, UK, and Gottfried emerged in pregnant rhesus monkeys and were transplacentally transmitted to the offspring. In the vaccine group, clinical symptoms of diarrhea, viral load in the gut tissue and histopathological changes were significantly reduced in the neonatal rhesus monkeys following oral challenge with the SA11 strain.
减毒活疫苗在欠发达国家或发展中国家的保护效果较低。然而，灭活轮状病毒疫苗可能有潜力克服其中的一些挑战。在本研究中，通过给新生恒河猴模型进行肠胃外给药，两价灭活轮状病毒疫苗的免疫原性和保护效力得到了提高。该两价灭活轮状病毒疫苗含有 G1P[8]（ZTR-68 株）和 G9P[8]（ZTR-18 株），在间隔 14 天的时间里给怀孕的恒河猴进行了两次给药。针对 RV 株 ZTR-68、ZTR-18、SA11、WA、UK 和 Gottfried 的中和抗体在怀孕的恒河猴中出现，并通过胎盘传递给后代。在疫苗组中，新生恒河猴在口服挑战 SA11 株后，腹泻的临床症状、肠道组织中的病毒载量和组织病理学变化均显著减轻。
